To clarify the intra- and inter-particle effects of magnesium ions on E. coli ribosomes we have performed measurements of light scattering intensity, index of refraction and small-angle neutron scattering on the 70S complex and 30S and 50S subunits with and without magnesium. The results indicate that magnesium has a specific intra-particle effect on the subunits as well as on the 70S complex. Besides, the distance distribution function shows that magnesium has an effect on the supra-ribosomal aggregation. The combination of these intra- and inter-particle effects completely hides, in the scattering experiments, any effect of magnesium on the degree of association of the two subunits into the 70S complex.
Structural change induced by removal of magnesium ions on E. coli 70S ribosomes and 30S and 50S separated subunits / Briganti, Giuseppe; Giansanti, Andrea; Bonincontro, Adalberto; M., Mengoni; R., Giordano. - In: JOURNAL OF MOLECULAR STRUCTURE. - ISSN 0022-2860. - 383:(1996), pp. 213-216. [10.1016/S0022-2860(96)09288-5]
Structural change induced by removal of magnesium ions on E. coli 70S ribosomes and 30S and 50S separated subunits
BRIGANTI, Giuseppe;GIANSANTI, Andrea;BONINCONTRO, Adalberto;
1996
Abstract
To clarify the intra- and inter-particle effects of magnesium ions on E. coli ribosomes we have performed measurements of light scattering intensity, index of refraction and small-angle neutron scattering on the 70S complex and 30S and 50S subunits with and without magnesium. The results indicate that magnesium has a specific intra-particle effect on the subunits as well as on the 70S complex. Besides, the distance distribution function shows that magnesium has an effect on the supra-ribosomal aggregation. The combination of these intra- and inter-particle effects completely hides, in the scattering experiments, any effect of magnesium on the degree of association of the two subunits into the 70S complex.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.