Glutamate decarboxylase (GAD) is a vitamin B6 enzyme which catalyzes the α-decarboxylation of l-glutamate to γ-aminobutyric acid (GABA). Escherichia coli cells coexpress two highly homologous enzyme isoforms, GADα and GADβ. Well diffracting crystals of GADβ were obtained by taking advantage of the possibility of expressing each isoform separately. They belong to space group P31 or P32 with the unit-cell dimensions a = b = 115.6 and c = 206.6 Å and contain one GAD hexamer in the asymmetric unit. High-resolution synchrotron data were collected at 100 K for the native protein and a potential heavy-atom derivative.
CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF THE BETA-ISOFORM OF GLUTAMATE DECARBOXYLASE FROM ESCHERICHIA COLI / Malashkevich, Vn; DE BIASE, Daniela; MARKOVIC HOUSLEY, Z; Schlunegger, Mp; Bossa, Francesco; Jansonius, Jn. - In: ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. - ISSN 0907-4449. - STAMPA. - D54:(1998), pp. 1020-1022. [10.1107/S0907444998003497]
CRYSTALLIZATION AND PRELIMINARY X-RAY ANALYSIS OF THE BETA-ISOFORM OF GLUTAMATE DECARBOXYLASE FROM ESCHERICHIA COLI.
DE BIASE, Daniela;BOSSA, Francesco;
1998
Abstract
Glutamate decarboxylase (GAD) is a vitamin B6 enzyme which catalyzes the α-decarboxylation of l-glutamate to γ-aminobutyric acid (GABA). Escherichia coli cells coexpress two highly homologous enzyme isoforms, GADα and GADβ. Well diffracting crystals of GADβ were obtained by taking advantage of the possibility of expressing each isoform separately. They belong to space group P31 or P32 with the unit-cell dimensions a = b = 115.6 and c = 206.6 Å and contain one GAD hexamer in the asymmetric unit. High-resolution synchrotron data were collected at 100 K for the native protein and a potential heavy-atom derivative.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
