The spectral behavior of calcein, a water-soluble self quenching fluorescent marker often used in biomedical analysis, can be considerably affected by the prescence of surfactants. With this study we intend to obtain further information on the photophysical properties of calcein, in the presence of surfactants and in the concentration range commonly used to investigate the release of such marker from vesicle dispersions. The experiments were carried out both in water and in a physiological buffer (HEPES, pH 7.5), in the presence of Triton X-100, sodium dodecyl sulphate and centyltrimethylammonium bromide, both below and above their critical micelle concentration (c.m.c.). The obtained results confirm that calcein flourescence can be affected by the presence of surfactants. Thus, environmental conditions must always be carefully checked for the actual quantitative evaluation of this dye. Furthermore, this study sheds some light on the nature and mechanism of calcein quenching.

Effects of surfactants on the spectral behaviour of calcein (II): A method of evaluation / Memoli, Adriana; M. G., Palermiti; Valter, Travagli; Alhaique, Franco. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 19:3-4(1999), pp. 627-632. [10.1016/s0731-7085(98)00229-5]

Effects of surfactants on the spectral behaviour of calcein (II): A method of evaluation

MEMOLI, Adriana;ALHAIQUE, Franco
1999

Abstract

The spectral behavior of calcein, a water-soluble self quenching fluorescent marker often used in biomedical analysis, can be considerably affected by the prescence of surfactants. With this study we intend to obtain further information on the photophysical properties of calcein, in the presence of surfactants and in the concentration range commonly used to investigate the release of such marker from vesicle dispersions. The experiments were carried out both in water and in a physiological buffer (HEPES, pH 7.5), in the presence of Triton X-100, sodium dodecyl sulphate and centyltrimethylammonium bromide, both below and above their critical micelle concentration (c.m.c.). The obtained results confirm that calcein flourescence can be affected by the presence of surfactants. Thus, environmental conditions must always be carefully checked for the actual quantitative evaluation of this dye. Furthermore, this study sheds some light on the nature and mechanism of calcein quenching.
1999
calcein; fluorescence changes; quenching; surfactants
01 Pubblicazione su rivista::01a Articolo in rivista
Effects of surfactants on the spectral behaviour of calcein (II): A method of evaluation / Memoli, Adriana; M. G., Palermiti; Valter, Travagli; Alhaique, Franco. - In: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS. - ISSN 0731-7085. - 19:3-4(1999), pp. 627-632. [10.1016/s0731-7085(98)00229-5]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/246903
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