The effect of cystathionine and cystathionine metabolites found in the urine of patients with cystathioninuria on the phosphorylation of tyrosine residues was studied with human peripheral blood polymorphonuclear leukocytes. Among the cystathionine metabolites, cystathionine ketimine markedly increased phosphorylation of a 45 kDa protein with time and the phosphorylation depended on the concentration of cystathionine ketimine, while cystathionine and the reduced form of cystathionine ketimine (cyclothionine) did not increase the phosphorylation of the 45 kDa protein. The phosphorylation of the 45 kDa protein induced by cystathionine ketimine was inhibited by genistein and herbimycin A, inhibitors of tyrosine kinase, but was not inhibited by 1-(5-isoquinolinesulfonyl)-2-methylpiperazine and staurosporine, inhibitors of protein kinase C. (C) 1996 Academic Press, Inc.

Effect of cystathionine and cystathionine metabolites on the phosphorylation of tyrosine residues in human neutrophils / Jianying, Zhang; K., Sugahara; Yasuhiro, Sagara; Fontana, Mario; Dupre', Silvestro; Cavallini, Doriano; Hiroyuki, Kodama. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 224:3(1996), pp. 849-854. [10.1006/bbrc.1996.1111]

Effect of cystathionine and cystathionine metabolites on the phosphorylation of tyrosine residues in human neutrophils

FONTANA, Mario;DUPRE', Silvestro;CAVALLINI, Doriano;
1996

Abstract

The effect of cystathionine and cystathionine metabolites found in the urine of patients with cystathioninuria on the phosphorylation of tyrosine residues was studied with human peripheral blood polymorphonuclear leukocytes. Among the cystathionine metabolites, cystathionine ketimine markedly increased phosphorylation of a 45 kDa protein with time and the phosphorylation depended on the concentration of cystathionine ketimine, while cystathionine and the reduced form of cystathionine ketimine (cyclothionine) did not increase the phosphorylation of the 45 kDa protein. The phosphorylation of the 45 kDa protein induced by cystathionine ketimine was inhibited by genistein and herbimycin A, inhibitors of tyrosine kinase, but was not inhibited by 1-(5-isoquinolinesulfonyl)-2-methylpiperazine and staurosporine, inhibitors of protein kinase C. (C) 1996 Academic Press, Inc.
1996
01 Pubblicazione su rivista::01a Articolo in rivista
Effect of cystathionine and cystathionine metabolites on the phosphorylation of tyrosine residues in human neutrophils / Jianying, Zhang; K., Sugahara; Yasuhiro, Sagara; Fontana, Mario; Dupre', Silvestro; Cavallini, Doriano; Hiroyuki, Kodama. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 224:3(1996), pp. 849-854. [10.1006/bbrc.1996.1111]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/246780
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 29
  • ???jsp.display-item.citation.isi??? 28
social impact