In in vitro studies, viable, intact human spermatozoa took up free radioinsulin with an apparently non-receptor-mediated mechanism. However, when a colloidal gold-insulin complex was substituted for the radiotracer, no surface binding was visualized at the ultrastructural level. Upon sperm incubation in the presence of free insulin, a dose-dependent release of phospholipid phosphorus occurred, with a concomitant derangement of head cell membrane. After head membrane removal, spermatozoa-bound radioinsulin in a time- and concentration-dependent manner, the binding was displaceable by unlabeled insulin, and an exclusive localization of the colloidal gold-insulin complex was visualized at the acrosome level. On the basis of this evidence, both the plasma membrane and the acrosome seem to represent cytological targets for insulin.

Insulin-sperm interaction: effects on plasma membrane and binding to acrosome / Silvestroni, Leopoldo; A., Modesti; Sartori, Claudia. - In: ARCHIVES OF ANDROLOGY. - ISSN 0148-5016. - STAMPA. - 28:(1992), pp. 201-211.

Insulin-sperm interaction: effects on plasma membrane and binding to acrosome

SILVESTRONI, Leopoldo;SARTORI, Claudia
1992

Abstract

In in vitro studies, viable, intact human spermatozoa took up free radioinsulin with an apparently non-receptor-mediated mechanism. However, when a colloidal gold-insulin complex was substituted for the radiotracer, no surface binding was visualized at the ultrastructural level. Upon sperm incubation in the presence of free insulin, a dose-dependent release of phospholipid phosphorus occurred, with a concomitant derangement of head cell membrane. After head membrane removal, spermatozoa-bound radioinsulin in a time- and concentration-dependent manner, the binding was displaceable by unlabeled insulin, and an exclusive localization of the colloidal gold-insulin complex was visualized at the acrosome level. On the basis of this evidence, both the plasma membrane and the acrosome seem to represent cytological targets for insulin.
1992
01 Pubblicazione su rivista::01a Articolo in rivista
Insulin-sperm interaction: effects on plasma membrane and binding to acrosome / Silvestroni, Leopoldo; A., Modesti; Sartori, Claudia. - In: ARCHIVES OF ANDROLOGY. - ISSN 0148-5016. - STAMPA. - 28:(1992), pp. 201-211.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/246543
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