Upon HPLC fractionation of human placenta or calf thymus H1 histone preparations, only some fractions enriched in the H1e-c variants were able to exert a severe inhibition on in vitro enzymatic DNA methylation. These fractions, though similar to the other variants in interacting with genomic DNA, were also the only ones which could bind CpG-rich ds-oligodeoxyribonucleotides (oligos). Both the 6-CpG ds-oligo and the DNA purified from chromatin fractions enriched in 'CpG islands' were good competitors for the binding of H1e-c to the 6meCpG ds-oligo. This ability to bind any DNA sequence and to suppress the enzymatic methylation in any sequence containing CpG dinucleotides suggests, for these particular H1 variants, a possible role in maintaining CpG island DNA and linker DNA at low methylation levels.

SPECIFIC VARIANTS OF H1 HISTONE REGULATE CPG METHYLATION IN EUKARYOTIC DNA / Strom, Roberto; Santoro, R.; D'Erme, Maria; Mastrantonio, S.; Reale, Anna; Marenzi, S.; Zardo, Giuseppe; Caiafa, Paola. - In: GENE. - ISSN 0378-1119. - STAMPA. - 157(1995), pp. 253-256. [10.1016/0378-1119(95)91236-S]

SPECIFIC VARIANTS OF H1 HISTONE REGULATE CPG METHYLATION IN EUKARYOTIC DNA.

STROM, Roberto;D'ERME, Maria;REALE, Anna;ZARDO, GIUSEPPE;CAIAFA, Paola
1995

Abstract

Upon HPLC fractionation of human placenta or calf thymus H1 histone preparations, only some fractions enriched in the H1e-c variants were able to exert a severe inhibition on in vitro enzymatic DNA methylation. These fractions, though similar to the other variants in interacting with genomic DNA, were also the only ones which could bind CpG-rich ds-oligodeoxyribonucleotides (oligos). Both the 6-CpG ds-oligo and the DNA purified from chromatin fractions enriched in 'CpG islands' were good competitors for the binding of H1e-c to the 6meCpG ds-oligo. This ability to bind any DNA sequence and to suppress the enzymatic methylation in any sequence containing CpG dinucleotides suggests, for these particular H1 variants, a possible role in maintaining CpG island DNA and linker DNA at low methylation levels.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11573/246036
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