Sorcin, a cytosolic calcium-binding protein containing a pair of EF-hand motifs, undergoes a Ca2+-dependent translocation to the cell membrane, The underlying conformational change is similar at pH 6.0 and 7.5 and consists in an increase in overall hydrophobicity that involves the aromatic residues and in particular the two tryptophan residues which become less exposed to solvent, The concomitant association from dimers to tetramers indicates that the tryptophan residues, which are located between the EF-hand sites, become buried at the dimer-dimer interface, Ca2+-bound sorcin displays a striking difference in solubility as a function of pH that has been ascribed to the formation of calcium-stabilized aggregates. (C) 1997 Federation of European Biochemical Societies.

Calcium- and pH-linked oligomerization of sorcin causing translocation from cytosol to membranes / Zamparelli, Carlotta; Ilari, Andrea; Daniela, Verzili; Paola, Vecchini; Chiancone, Emilia. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 409:1(1997), pp. 1-6. [10.1016/s0014-5793(97)00464-x]

Calcium- and pH-linked oligomerization of sorcin causing translocation from cytosol to membranes

ZAMPARELLI, Carlotta;ILARI, ANDREA;CHIANCONE, Emilia
1997

Abstract

Sorcin, a cytosolic calcium-binding protein containing a pair of EF-hand motifs, undergoes a Ca2+-dependent translocation to the cell membrane, The underlying conformational change is similar at pH 6.0 and 7.5 and consists in an increase in overall hydrophobicity that involves the aromatic residues and in particular the two tryptophan residues which become less exposed to solvent, The concomitant association from dimers to tetramers indicates that the tryptophan residues, which are located between the EF-hand sites, become buried at the dimer-dimer interface, Ca2+-bound sorcin displays a striking difference in solubility as a function of pH that has been ascribed to the formation of calcium-stabilized aggregates. (C) 1997 Federation of European Biochemical Societies.
1997
calcium and ph dependent conformational change; calcium- and ph-dependent conformational change; oligomerization; sorcin
01 Pubblicazione su rivista::01a Articolo in rivista
Calcium- and pH-linked oligomerization of sorcin causing translocation from cytosol to membranes / Zamparelli, Carlotta; Ilari, Andrea; Daniela, Verzili; Paola, Vecchini; Chiancone, Emilia. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 409:1(1997), pp. 1-6. [10.1016/s0014-5793(97)00464-x]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/245876
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 28
  • ???jsp.display-item.citation.isi??? 26
social impact