A protocol for in vitro plant regeneration through organogenesis was established for Vismia guianensis (Hypericaceae), a species that produces an anti-cancer compound. The highest mean number of shoots per gram of callus (57.33) was obtained on Murashige and Skoog medium supplemented with 4.44 mu M 6-benzyl-aminopurine, 5.70 mu M indole-3-acetic acid and 12.88 mu M gibberellic acid. Rooting was favoured by the addition of 10 mu M indole-3-butyric acid, and by sucrose concentrations higher than 1%.
In vitro plant regeneration of Vismia guianensis through organogenesis / Monacelli, Barbara; Pasqua, Gabriella; A., Cuteri; A., Vitali. - In: PLANT CELL TISSUE AND ORGAN CULTURE. - ISSN 0167-6857. - 58:2(1999), pp. 81-85. [10.1023/a:1006381307764]
In vitro plant regeneration of Vismia guianensis through organogenesis
MONACELLI, Barbara;PASQUA, Gabriella;
1999
Abstract
A protocol for in vitro plant regeneration through organogenesis was established for Vismia guianensis (Hypericaceae), a species that produces an anti-cancer compound. The highest mean number of shoots per gram of callus (57.33) was obtained on Murashige and Skoog medium supplemented with 4.44 mu M 6-benzyl-aminopurine, 5.70 mu M indole-3-acetic acid and 12.88 mu M gibberellic acid. Rooting was favoured by the addition of 10 mu M indole-3-butyric acid, and by sucrose concentrations higher than 1%.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
