Background - Previous studies have shown that cirrhotic patients produce increased amounts of thrombin but the underlying mechanism is still unknown. Aims - To analyse the relation between the rate of thrombin generation and monocyte expression of tissue factor (TF) in cirrhosis. Patients - Thirty three cirrhotic patients classified as having low (n = 7), moderate (n = 17), or severe (n = 9) liver failure according to Child-Pugh criteria. Methods - Prothrombin fragment F1+2, monocyte TF activity and antigen, and endotoxaemia were measured in all patients. Polymerase chain reaction (PCR) analysis of TF mRNA was performed in monocytes of five cirrhotic patients. Results - Prothrombin fragment F1+2 was higher in cirrhotic patients,than in controls (p < 0.0001). Monocytes from cirrhotic patients had higher TF activity and antigen than those from controls (p < 0.001) with a progressive increase from low to severe liver failure. Monocyte expression of TF was significantly correlated with plasma levels of F1+2 (TF activity: r = 0.98, p < 0.0001; TF antigen: r = 0.95, p < 0.0001) and with endotoxaemia (TF activity: r = 0.94, p < 0.0001; TF antigen: r = 0.91, p < 0.0001). PCR analysis of TF mRNA showed TF expression only in three patients with endotoxaemia (more than 15 pg/ml). Conclusions - Cirrhotic patients have enhanced expression of TF which could be responsible for clotting activation, suggesting that endotoxaemia might play a pivotal role.

Enhanced expression of monocyte tissue factor in patients with liver cirrhosis / Saliola, Mirella; R., Lorenzet; Ferro, Domenico; Basili, Stefania; C., Caroselli; Santo, Ad; M., Sallese; Violi, Francesco. - In: GUT. - ISSN 0017-5749. - 43:3(1998), pp. 428-432. [10.1136/gut.43.3.428]

Enhanced expression of monocyte tissue factor in patients with liver cirrhosis

SALIOLA, Mirella;FERRO, Domenico;BASILI, Stefania;VIOLI, Francesco
1998

Abstract

Background - Previous studies have shown that cirrhotic patients produce increased amounts of thrombin but the underlying mechanism is still unknown. Aims - To analyse the relation between the rate of thrombin generation and monocyte expression of tissue factor (TF) in cirrhosis. Patients - Thirty three cirrhotic patients classified as having low (n = 7), moderate (n = 17), or severe (n = 9) liver failure according to Child-Pugh criteria. Methods - Prothrombin fragment F1+2, monocyte TF activity and antigen, and endotoxaemia were measured in all patients. Polymerase chain reaction (PCR) analysis of TF mRNA was performed in monocytes of five cirrhotic patients. Results - Prothrombin fragment F1+2 was higher in cirrhotic patients,than in controls (p < 0.0001). Monocytes from cirrhotic patients had higher TF activity and antigen than those from controls (p < 0.001) with a progressive increase from low to severe liver failure. Monocyte expression of TF was significantly correlated with plasma levels of F1+2 (TF activity: r = 0.98, p < 0.0001; TF antigen: r = 0.95, p < 0.0001) and with endotoxaemia (TF activity: r = 0.94, p < 0.0001; TF antigen: r = 0.91, p < 0.0001). PCR analysis of TF mRNA showed TF expression only in three patients with endotoxaemia (more than 15 pg/ml). Conclusions - Cirrhotic patients have enhanced expression of TF which could be responsible for clotting activation, suggesting that endotoxaemia might play a pivotal role.
monocyte; prothrombin fragment f1+2; tissue factor
01 Pubblicazione su rivista::01a Articolo in rivista
Enhanced expression of monocyte tissue factor in patients with liver cirrhosis / Saliola, Mirella; R., Lorenzet; Ferro, Domenico; Basili, Stefania; C., Caroselli; Santo, Ad; M., Sallese; Violi, Francesco. - In: GUT. - ISSN 0017-5749. - 43:3(1998), pp. 428-432. [10.1136/gut.43.3.428]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/245037
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