A rabbit liver cDNA library in phage lambda gt10 was screened using the coding cDNA for human cytosolic serine hydroxymethyltransferase. A clone of 1754 bp was isolated and the nucleotide sequence showed an open reading frame of 1455 bp, which coded for rabbit cytosolic serine hydroxymethyltransferase and was flanked by 12 bp at the 5' end and 287 bp at the 3' end. The full-length cDNA was then cloned into a pET22b vector as a NdeI-EcoRI insert. HMS174(DE3) cells were transformed with this plasmid and, after induction with isopropyl beta-D-thiogalactopyranoside, expressed a catalytically active serine hydroxymethyltransferase. The enzyme was purified and shown to be the expressed rabbit enzyme lacking the first methionine residue. Spectral characteristics of the bound pyridoxal phosphate and kinetic constants for the natural substrates L-serine and tetrahydrofolate were essentially identical to the values obtained previously for the rabbit cytosolic enzyme. The pattern of bands shown by the pure recombinant enzyme on an isoelectric focusing gel containing 6 M urea showed a major band and a minor band representing about 15-20% of the protein. Upon incubation of the recombinant enzyme at pH 7.3 and 37 degrees C, three new bands were observed on isoelectric focusing with the concomitant formation of isoaspartyl residues, as determined by reactivity with protein isoaspartyl methyltransferase. These results are consistent with deamidation of Asn residues to isoaspartyl during the in vitro incubation. The enzyme purified from rabbit liver has previously been shown to contain isoaspartyl residues. (C) 1998 Academic Press.

Purification and characterization of recombinant rabbit cytosolic serine hydroxymethyltransferase / DI SALVO, Martino Luigi; S., Delle Fratte; DE BIASE, Daniela; Bossa, Francesco; Verne, Schirch. - In: PROTEIN EXPRESSION AND PURIFICATION. - ISSN 1046-5928. - STAMPA. - 13:2(1998), pp. 177-183. [10.1006/prep.1998.0890]

Purification and characterization of recombinant rabbit cytosolic serine hydroxymethyltransferase

DI SALVO, Martino Luigi;DE BIASE, Daniela;BOSSA, Francesco;
1998

Abstract

A rabbit liver cDNA library in phage lambda gt10 was screened using the coding cDNA for human cytosolic serine hydroxymethyltransferase. A clone of 1754 bp was isolated and the nucleotide sequence showed an open reading frame of 1455 bp, which coded for rabbit cytosolic serine hydroxymethyltransferase and was flanked by 12 bp at the 5' end and 287 bp at the 3' end. The full-length cDNA was then cloned into a pET22b vector as a NdeI-EcoRI insert. HMS174(DE3) cells were transformed with this plasmid and, after induction with isopropyl beta-D-thiogalactopyranoside, expressed a catalytically active serine hydroxymethyltransferase. The enzyme was purified and shown to be the expressed rabbit enzyme lacking the first methionine residue. Spectral characteristics of the bound pyridoxal phosphate and kinetic constants for the natural substrates L-serine and tetrahydrofolate were essentially identical to the values obtained previously for the rabbit cytosolic enzyme. The pattern of bands shown by the pure recombinant enzyme on an isoelectric focusing gel containing 6 M urea showed a major band and a minor band representing about 15-20% of the protein. Upon incubation of the recombinant enzyme at pH 7.3 and 37 degrees C, three new bands were observed on isoelectric focusing with the concomitant formation of isoaspartyl residues, as determined by reactivity with protein isoaspartyl methyltransferase. These results are consistent with deamidation of Asn residues to isoaspartyl during the in vitro incubation. The enzyme purified from rabbit liver has previously been shown to contain isoaspartyl residues. (C) 1998 Academic Press.
1998
cdna library; isoaspartyl residues; plp-dependent enzymes; protein overexpression; serine hydroxymethyltransferase
01 Pubblicazione su rivista::01a Articolo in rivista
Purification and characterization of recombinant rabbit cytosolic serine hydroxymethyltransferase / DI SALVO, Martino Luigi; S., Delle Fratte; DE BIASE, Daniela; Bossa, Francesco; Verne, Schirch. - In: PROTEIN EXPRESSION AND PURIFICATION. - ISSN 1046-5928. - STAMPA. - 13:2(1998), pp. 177-183. [10.1006/prep.1998.0890]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/245005
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