To evaluate the role of the acidic pH of phagosomes on the invasive ability and fate of Listeria monocytogenes within host cells, entry and replication of this gram-positive bacterium in a human enterocyte-like cell line (Caco-2) were investigated by a combination of biochemical and ultrastructural approaches, The effects of inhibitors of vacuolar acidification - the lipophilic weak base ammonium chloride, the carboxylic ionophore monensin and the vacuolar proton ATPase inhibitor bafilomycin A(1) - on the bacterial invasion pathway were analysed, These agents, which raise the intracellular vesicle acidic pH of living cells by different mechanisms, affected L. monocytogenes replication in Caco-2 cells, Bacteria internalised by bafilomycin-treated cells were unable to escape from phagosomes, as demonstrated by electronmicroscopy. The results provide evidence that low pH is required for efficient intracellular growth of L. monocytogenes.

The effects of inhibitors of vacuolar acidification on the release of Listeria monocytogenes from phagosomes of Caco-2 cells / Conte, Maria Pia; G., Petrone; Longhi, Catia; Valenti, Piera; R., Morelli; F., Superti; Seganti, Lucilla. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 44:6(1996), pp. 418-424. [10.1099/00222615-44-6-418]

The effects of inhibitors of vacuolar acidification on the release of Listeria monocytogenes from phagosomes of Caco-2 cells

CONTE, Maria Pia;LONGHI, Catia;VALENTI, PIERA;SEGANTI, Lucilla
1996

Abstract

To evaluate the role of the acidic pH of phagosomes on the invasive ability and fate of Listeria monocytogenes within host cells, entry and replication of this gram-positive bacterium in a human enterocyte-like cell line (Caco-2) were investigated by a combination of biochemical and ultrastructural approaches, The effects of inhibitors of vacuolar acidification - the lipophilic weak base ammonium chloride, the carboxylic ionophore monensin and the vacuolar proton ATPase inhibitor bafilomycin A(1) - on the bacterial invasion pathway were analysed, These agents, which raise the intracellular vesicle acidic pH of living cells by different mechanisms, affected L. monocytogenes replication in Caco-2 cells, Bacteria internalised by bafilomycin-treated cells were unable to escape from phagosomes, as demonstrated by electronmicroscopy. The results provide evidence that low pH is required for efficient intracellular growth of L. monocytogenes.
1996
01 Pubblicazione su rivista::01a Articolo in rivista
The effects of inhibitors of vacuolar acidification on the release of Listeria monocytogenes from phagosomes of Caco-2 cells / Conte, Maria Pia; G., Petrone; Longhi, Catia; Valenti, Piera; R., Morelli; F., Superti; Seganti, Lucilla. - In: JOURNAL OF MEDICAL MICROBIOLOGY. - ISSN 0022-2615. - 44:6(1996), pp. 418-424. [10.1099/00222615-44-6-418]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/244887
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