DNA-protein cross-linkages were performed in intact undifferentiated and differentiated-HL60 cells by the action of cis-diammine dichloroplatinum. Total nuclear matrix proteins and DNA cross-linked nuclear matrix proteins were resolved by two-dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of a set of differentiation-induced nuclear matrix proteins cross-linked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and characterizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differentiation.
Differentation-specific nuclear matrix proteins cross-linked to DNA by cis-diamminedichloroplatinum / Mattia, Elena; Eufemi, Margherita; Chichiarelli, Silvia; Ceridono, M:; Ferraro, Anna. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 238:(1998), pp. 216-219. [10.1006/excr.1997.3833]
Differentation-specific nuclear matrix proteins cross-linked to DNA by cis-diamminedichloroplatinum.
MATTIA, Elena;EUFEMI, Margherita;CHICHIARELLI, Silvia;FERRARO, Anna
1998
Abstract
DNA-protein cross-linkages were performed in intact undifferentiated and differentiated-HL60 cells by the action of cis-diammine dichloroplatinum. Total nuclear matrix proteins and DNA cross-linked nuclear matrix proteins were resolved by two-dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of a set of differentiation-induced nuclear matrix proteins cross-linked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and characterizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differentiation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.