A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples

"Determination of arginosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography" / Canepari, Silvia; Carunchio, Vincenzo Tito; Girelli, Anna Maria; Messina, Antonella. - In: BIOMEDICAL CHROMATOGRAPHY. - ISSN 0269-3879. - STAMPA. - 9:(1995), pp. 171-174. [10.1002/bmc.1130090404]

"Determination of arginosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography"

CANEPARI, Silvia;CARUNCHIO, Vincenzo Tito;GIRELLI, Anna Maria;MESSINA, Antonella
1995

Abstract

A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples
1995
01 Pubblicazione su rivista::01a Articolo in rivista
"Determination of arginosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography" / Canepari, Silvia; Carunchio, Vincenzo Tito; Girelli, Anna Maria; Messina, Antonella. - In: BIOMEDICAL CHROMATOGRAPHY. - ISSN 0269-3879. - STAMPA. - 9:(1995), pp. 171-174. [10.1002/bmc.1130090404]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/243693
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 1
  • ???jsp.display-item.citation.isi??? 1
social impact