A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samples
"Determination of arginosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography" / Canepari, Silvia; Carunchio, Vincenzo Tito; Girelli, Anna Maria; Messina, Antonella. - In: BIOMEDICAL CHROMATOGRAPHY. - ISSN 0269-3879. - STAMPA. - 9:(1995), pp. 171-174. [10.1002/bmc.1130090404]
"Determination of arginosuccinate lyase in human serum by ion-pair reversed phase liquid chromatography"
CANEPARI, Silvia;CARUNCHIO, Vincenzo Tito;GIRELLI, Anna Maria;MESSINA, Antonella
1995
Abstract
A new assay for argininosuccinate lyase based on the separation of the enzymatic reaction mixture components by an ion-pair reversed phase mechanism is reported. The determination of enzyme activity is performed after direct injection by UV detection of the fumarate formed. The chromatographic analysis time is 8 min and a detection limit of 0.4 U/L is achieved. The HPLC method is highly accurate, sensitive and precise. The simple procedure makes this method suitable for the routine determination of ASAL activity in human serum samplesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.