An experimental investigation was carried out on the influence of some hydroxylic additives (up to 30% by weight) on the thermal stability of erythrocyte carbonic anhydrase. The melting temperatures of the protein in the different media were determined by spectroscopic measurements. Xylitol and maltose stabilized the enzyme, whereas ethanol, 1-propanol, ethanediol and 1,2-propanediol caused a destabilization of the protein. In all the systems examined, the transition temperature was found to be strictly related to the surface tension of the medium. A molecular thermodynamic model was developed, based on the chemical equilibrium between the native and denatured protein forms, which enabled to interpret the observed behaviour.

Effect of surface tension on the conformational stability of erythrocyte carbonic anhydrase / Federico, Cioci; Lavecchia, Roberto; Marrelli, Luigi. - In: FLUID PHASE EQUILIBRIA. - ISSN 0378-3812. - 116:1-2(1996), pp. 118-125. (Intervento presentato al convegno 7th International Conference on Fluid Properties and Phase Equilibria for Chemical Process Design tenutosi a SNOWMASS VILLAGE, CO nel JUN 18-23, 1995) [10.1016/0378-3812(95)02879-x].

Effect of surface tension on the conformational stability of erythrocyte carbonic anhydrase

LAVECCHIA, Roberto;MARRELLI, Luigi
1996

Abstract

An experimental investigation was carried out on the influence of some hydroxylic additives (up to 30% by weight) on the thermal stability of erythrocyte carbonic anhydrase. The melting temperatures of the protein in the different media were determined by spectroscopic measurements. Xylitol and maltose stabilized the enzyme, whereas ethanol, 1-propanol, ethanediol and 1,2-propanediol caused a destabilization of the protein. In all the systems examined, the transition temperature was found to be strictly related to the surface tension of the medium. A molecular thermodynamic model was developed, based on the chemical equilibrium between the native and denatured protein forms, which enabled to interpret the observed behaviour.
1996
01 Pubblicazione su rivista::01a Articolo in rivista
Effect of surface tension on the conformational stability of erythrocyte carbonic anhydrase / Federico, Cioci; Lavecchia, Roberto; Marrelli, Luigi. - In: FLUID PHASE EQUILIBRIA. - ISSN 0378-3812. - 116:1-2(1996), pp. 118-125. (Intervento presentato al convegno 7th International Conference on Fluid Properties and Phase Equilibria for Chemical Process Design tenutosi a SNOWMASS VILLAGE, CO nel JUN 18-23, 1995) [10.1016/0378-3812(95)02879-x].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/243588
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