We have developed a new experimental approach, Temperature Derivative X-ray Absorption Spectroscopy (TDXAS), to measure the conformational landscape of a metalloprotein and how it depends on the selected conformational coordinate of the metal active site structure. We have recorded the temperature variation of a selected feature of the X ray Absorption Near Edge Structure (XANES) at the iron K edge of carbonmonoxy-myoglobin (MbCO) of a photoproduct and we have extracted the distribution functions g(H) of activation enthalpy barriers H for CO recombination for different conformational coordinates.
TDXAS study of the conformational landscapeof MbCO / Bianconi, Antonio; F., Natali; M. L., Alosi; S., Grande; A., Lanzara; Saini, Naurang Lal; M., Brunelli. - In: JOURNAL OF SYNCHROTRON RADIATION. - ISSN 0909-0495. - STAMPA. - 6:(1999), pp. 389-391. [10.1107/S0909049598017063]
TDXAS study of the conformational landscapeof MbCO
BIANCONI, Antonio;SAINI, Naurang Lal;
1999
Abstract
We have developed a new experimental approach, Temperature Derivative X-ray Absorption Spectroscopy (TDXAS), to measure the conformational landscape of a metalloprotein and how it depends on the selected conformational coordinate of the metal active site structure. We have recorded the temperature variation of a selected feature of the X ray Absorption Near Edge Structure (XANES) at the iron K edge of carbonmonoxy-myoglobin (MbCO) of a photoproduct and we have extracted the distribution functions g(H) of activation enthalpy barriers H for CO recombination for different conformational coordinates.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.