The sequence of a gene from Bombina orientalis was determined which codes for antibacterial peptides. The gene comprises two exons separated by a large intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for two identical bombinins and one bombinin H. The promoter region of the bombinin gene contains putative recognition sites for nuclear factors, such as NFκB and NF-IL6. In vivo experiments on B. orientalis have shown that a short contact with bacteria is sufficient to induce a marked increase in the amount of antibacterial peptides in the skin secretion of frogs. This increase was suppressed by pretreatment with glucocorticoids. In the latter case, a significant increase of IκBα in the secretion is also detectable.

MOLECULAR CLONING OF A BOMBININ GENE FROM BOMBINA ORIENTALIS: DETECTION OF NF-kappaB AND NF-IL6 BINDING SITES IN ITS PROMOTER / Miele, Rossella; Ponti, Donatella; Boman, Hg; Barra, Donatella; Simmaco, Maurizio. - In: FEBS LETTERS. - ISSN 0014-5793. - 431:(1998), pp. 23-28. [10.1016/S0014-5793(98)00718-2]

MOLECULAR CLONING OF A BOMBININ GENE FROM BOMBINA ORIENTALIS: DETECTION OF NF-kappaB AND NF-IL6 BINDING SITES IN ITS PROMOTER

MIELE, Rossella;PONTI, Donatella;BARRA, Donatella;SIMMACO, Maurizio
1998

Abstract

The sequence of a gene from Bombina orientalis was determined which codes for antibacterial peptides. The gene comprises two exons separated by a large intron. Exon 1 codes for the signal peptide, while exon 2 contains the genetic information for two identical bombinins and one bombinin H. The promoter region of the bombinin gene contains putative recognition sites for nuclear factors, such as NFκB and NF-IL6. In vivo experiments on B. orientalis have shown that a short contact with bacteria is sufficient to induce a marked increase in the amount of antibacterial peptides in the skin secretion of frogs. This increase was suppressed by pretreatment with glucocorticoids. In the latter case, a significant increase of IκBα in the secretion is also detectable.
1998
01 Pubblicazione su rivista::01a Articolo in rivista
MOLECULAR CLONING OF A BOMBININ GENE FROM BOMBINA ORIENTALIS: DETECTION OF NF-kappaB AND NF-IL6 BINDING SITES IN ITS PROMOTER / Miele, Rossella; Ponti, Donatella; Boman, Hg; Barra, Donatella; Simmaco, Maurizio. - In: FEBS LETTERS. - ISSN 0014-5793. - 431:(1998), pp. 23-28. [10.1016/S0014-5793(98)00718-2]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/243242
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