The conformation of oxidized scleroglucan (sclerox) was characterized in water and in NaCl aqueous solution by means of small-angle X-ray scattering (SAXS). The observed SAXS profiles were analyzed in terms of a modified broken rod model, where each rod component was replaced with the molecular models of a triple-stranded helix and a single chain simulated by the Monte Carlo method. The electrostatic interaction due to carboxylate groups was taken into account by assuming a Gaussian-type interaction potential. The results confirmed that the triple-stranded helix of scleroglucan was disentangled to a single chain by the oxidation of β-D-glucosyl side residues. The triple-stranded structure is partly recovered by shielding charge on the side chains.
Conformational characteristics of oxidized scleroglucan / Coviello, Tommasina; Hitoshi, Maeda; Y., Yuguchui; Hiroshi, Urakawa; Kanji, Kajiwara; Dentini, Mariella; Crescenzi, Vittorio. - In: MACROMOLECULES. - ISSN 0024-9297. - 31:5(1998), pp. 1602-1607. [10.1021/ma9716286]
Conformational characteristics of oxidized scleroglucan
COVIELLO, Tommasina;DENTINI, Mariella;CRESCENZI, Vittorio
1998
Abstract
The conformation of oxidized scleroglucan (sclerox) was characterized in water and in NaCl aqueous solution by means of small-angle X-ray scattering (SAXS). The observed SAXS profiles were analyzed in terms of a modified broken rod model, where each rod component was replaced with the molecular models of a triple-stranded helix and a single chain simulated by the Monte Carlo method. The electrostatic interaction due to carboxylate groups was taken into account by assuming a Gaussian-type interaction potential. The results confirmed that the triple-stranded helix of scleroglucan was disentangled to a single chain by the oxidation of β-D-glucosyl side residues. The triple-stranded structure is partly recovered by shielding charge on the side chains.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
