The phenomenon of posttranscriptional gene silencing (PTGS), which occurs when a transgene is introduced into a cell, is poorly understood. Here, the qde-3 gene, which is required for the activation and maintenance of gene silencing in the fungus Neurospora crassa, was isolated. Sequence analysis revealed that the qde-3 gene belongs to the RecQ DNA helicase family. The QDE3 protein may function in the DNA-DNA interaction between introduced transgenes or with an endogenous gene required for gene-silencing activation. In animals, genes that are homologous to RecQ protein, such as the human genes for Bloom's syndrome and Werner's syndrome, may also function in PTGS.

Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase / Cogoni, Carlo; Macino, Giuseppe. - In: SCIENCE. - ISSN 0036-8075. - 286:5448(1999), pp. 2342-2344. [10.1126/science.286.5448.2342]

Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase

COGONI, Carlo;MACINO, Giuseppe
1999

Abstract

The phenomenon of posttranscriptional gene silencing (PTGS), which occurs when a transgene is introduced into a cell, is poorly understood. Here, the qde-3 gene, which is required for the activation and maintenance of gene silencing in the fungus Neurospora crassa, was isolated. Sequence analysis revealed that the qde-3 gene belongs to the RecQ DNA helicase family. The QDE3 protein may function in the DNA-DNA interaction between introduced transgenes or with an endogenous gene required for gene-silencing activation. In animals, genes that are homologous to RecQ protein, such as the human genes for Bloom's syndrome and Werner's syndrome, may also function in PTGS.
1999
01 Pubblicazione su rivista::01a Articolo in rivista
Posttranscriptional gene silencing in Neurospora by a RecQ DNA helicase / Cogoni, Carlo; Macino, Giuseppe. - In: SCIENCE. - ISSN 0036-8075. - 286:5448(1999), pp. 2342-2344. [10.1126/science.286.5448.2342]
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/242547
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 81
  • Scopus 221
  • ???jsp.display-item.citation.isi??? 204
social impact