We describe a model for the three-dimensional structure of E. coli serine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are known. The model suggests that certain amino acid residues at the putative active site of the enzyme can adopt specific roles in the catalytic mechanism. These proposals were supported by analysis of the properties of a number of site-directed mutants. New active site features are also proposed for further experimental testing.

The structure of serine hydrxymethyltransferase as modeled by homology and validated by site-directed mutagenesis / Pascarella, Stefano; Angelaccio, Sebastiana; Contestabile, Roberto; DELLE FRATTE, S; DI SALVO, Martino Luigi; Bossa, Francesco. - In: PROTEIN SCIENCE. - ISSN 0961-8368. - STAMPA. - 7:(1998), pp. 1976-1982. [10.1002/pro.5560070913]

The structure of serine hydrxymethyltransferase as modeled by homology and validated by site-directed mutagenesis

PASCARELLA, Stefano;ANGELACCIO, Sebastiana;CONTESTABILE, Roberto;DI SALVO, Martino Luigi;BOSSA, Francesco
1998

Abstract

We describe a model for the three-dimensional structure of E. coli serine hydroxymethyltransferase based on its sequence homology with other PLP enzymes of the alpha-family and whose tertiary structures are known. The model suggests that certain amino acid residues at the putative active site of the enzyme can adopt specific roles in the catalytic mechanism. These proposals were supported by analysis of the properties of a number of site-directed mutants. New active site features are also proposed for further experimental testing.
1998
ASPARTATE AMINOTRANSFERASE; CATALYSIS; Evolution; HOMOLOGY MODELING; Profile analysis; Pyridoxal phosphate; SERINE HYDROXYMETHYLTRANSFERASE; site-directed mutagenesis
01 Pubblicazione su rivista::01a Articolo in rivista
The structure of serine hydrxymethyltransferase as modeled by homology and validated by site-directed mutagenesis / Pascarella, Stefano; Angelaccio, Sebastiana; Contestabile, Roberto; DELLE FRATTE, S; DI SALVO, Martino Luigi; Bossa, Francesco. - In: PROTEIN SCIENCE. - ISSN 0961-8368. - STAMPA. - 7:(1998), pp. 1976-1982. [10.1002/pro.5560070913]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/242011
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