H1e and H1c histone variants were purified from mouse L929 fibroblasts using a reverse phase HPLC, and their effect on in vitro DNA methylation was investigated, together with their ability to bind unmethylated or methylated CpG-rich 44bp oligonucleotides. In a "physiological" range of H1:DNA ratios only H1e, at variance from H1c, was found to cause a marked inhibition of in vitro enzymic DNA methylation. It was also shown that both variants have a similar affinity in binding a methylated CpG-rich oligonucleotide, but that the binding to the same oligonucleotide in the unmethylated form occurs preferentially with H1e rather than with H1c. H1e is therefore likely to be directly involved in maintaining CpG-rich sequences in the unmethylated state.

Specific inhibitory effect of H1e histone somatic variant on in vitro DNA methylation process / Zardo, Giuseppe; Santoro, R.; D'Erme, Maria; Reale, Anna; Guidobaldi, L:; Caiafa, Paola; Strom, Roberto. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 220:(1996), pp. 102-107. [10.1006/bbrc.1996.0364]

Specific inhibitory effect of H1e histone somatic variant on in vitro DNA methylation process

ZARDO, GIUSEPPE;D'ERME, Maria;REALE, Anna;CAIAFA, Paola;STROM, Roberto
1996

Abstract

H1e and H1c histone variants were purified from mouse L929 fibroblasts using a reverse phase HPLC, and their effect on in vitro DNA methylation was investigated, together with their ability to bind unmethylated or methylated CpG-rich 44bp oligonucleotides. In a "physiological" range of H1:DNA ratios only H1e, at variance from H1c, was found to cause a marked inhibition of in vitro enzymic DNA methylation. It was also shown that both variants have a similar affinity in binding a methylated CpG-rich oligonucleotide, but that the binding to the same oligonucleotide in the unmethylated form occurs preferentially with H1e rather than with H1c. H1e is therefore likely to be directly involved in maintaining CpG-rich sequences in the unmethylated state.
1996
Epigenetics; Histone variants; DNA methylation
01 Pubblicazione su rivista::01a Articolo in rivista
Specific inhibitory effect of H1e histone somatic variant on in vitro DNA methylation process / Zardo, Giuseppe; Santoro, R.; D'Erme, Maria; Reale, Anna; Guidobaldi, L:; Caiafa, Paola; Strom, Roberto. - In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. - ISSN 0006-291X. - STAMPA. - 220:(1996), pp. 102-107. [10.1006/bbrc.1996.0364]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/241760
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