The existence of a possible correlation between poly(ADP-ribosyl)ation and DNA methylation processes was investigated. In vivo and in vitro experiments were carried out on L929 mouse fibroblasts preincubated for 24 h with or without 3-aminobenzamide, a well-known inhibitor of poly(ADP-ribose) polymerase. Both experimental approaches evidenced a close relationship between these two important nuclear enzymatic mechanisms, suggesting that the poly(ADP-ribosyl)ated isoform of H1 histone and/or long and branched protein-free ADP-ribose polymers could act as protecting agents against full methylation of the CpG dinucleotides in genomic DNA.
Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern? / Zardo, Giuseppe; D'Erme, Maria; Reale, Anna; Strom, Roberto; Perilli, M; Caiafa, Paola. - In: BIOCHEMISTRY. - ISSN 0006-2960. - STAMPA. - 36:(1997), pp. 7937-7943. [10.1021/bi970241s]
Does poly(ADP-ribosyl)ation regulate the DNA methylation pattern?
ZARDO, GIUSEPPE;D'ERME, Maria;REALE, Anna;STROM, Roberto;CAIAFA, Paola
1997
Abstract
The existence of a possible correlation between poly(ADP-ribosyl)ation and DNA methylation processes was investigated. In vivo and in vitro experiments were carried out on L929 mouse fibroblasts preincubated for 24 h with or without 3-aminobenzamide, a well-known inhibitor of poly(ADP-ribose) polymerase. Both experimental approaches evidenced a close relationship between these two important nuclear enzymatic mechanisms, suggesting that the poly(ADP-ribosyl)ated isoform of H1 histone and/or long and branched protein-free ADP-ribose polymers could act as protecting agents against full methylation of the CpG dinucleotides in genomic DNA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
