A typical feature of lupus nephritis is glomerular and interstitial leukocyte infiltration. In search of a serological marker of renal disease activity, we examined prostaglandin endoperoxide synthetase (PGHS) activity in peripheral-blood monocytes isolated from 5 healthy subjects and 11 untreated patients with biopsy-proven lupus nephritis, using radioimmunoassay of prostaglandin E-2 (PGE(2)) and thromboxane B-2 (TxB(2)) released during 24-hour cultures with selective stimuli/inhibitors. Unstimulated basal PGE(2) and TxB(2) synthesis, reflecting in vivo PGHS activity, was greater in the five patients with active renal involvement (World Health Organization [WHO] classes IVb-c) and the six lupus patients without active disease than in the five healthy subjects (TxB(2), 2,643 +/- 198 [standard error], 2,015 +/- 190, 1,548 +/- 295 pg/10(6) cells, respectively). Escherichia coli lipopolysaccharide (LPS; 10 mu g/mL) potently induced TxB(2) or PGE(2) synthesis in healthy controls (+255% +/- 76% and +611% +/- 190%, +688% +/- 234% and +3,189% +/- 154%; 4 to 24 hours, respectively), an effect abolished by 5 mu mol/L of dexamethasone (DEX) or by 5 mu mol/L of the protein synthesis inhibitor cycloheximide (CHX). Responses to LPS were reduced in lupus patients without disease activity and reduced even further in those with active nephritis. This may be related to substrate depletion or feedback functional inhibition of the inducible isoform of PGHS. Our assay may prove useful in the early detection of kidney disease activity in lupus erythematosus. (C) 1998 by the National Kidney Foundation, Inc.

Eicosanoid synthesis in peripheral blood monocytes: A marker of disease activity in lupus nephritis / Mene', Paolo; Pecci, Gabriella; Cinotti, Giulio Alberto; Pugliese, Giuseppe; F., Pricci; Pugliese, Francesco. - In: AMERICAN JOURNAL OF KIDNEY DISEASES. - ISSN 0272-6386. - 32:5(1998), pp. 778-784. (Intervento presentato al convegno 29th Annual Meeting of the American-Society-of-Nephrology tenutosi a NEW ORLEANS, LOUISIANA nel NOV 03-06, 1996) [10.1016/s0272-6386(98)70133-7].

Eicosanoid synthesis in peripheral blood monocytes: A marker of disease activity in lupus nephritis

MENE', Paolo;PECCI, Gabriella;CINOTTI, Giulio Alberto;PUGLIESE, Giuseppe;PUGLIESE, Francesco
1998

Abstract

A typical feature of lupus nephritis is glomerular and interstitial leukocyte infiltration. In search of a serological marker of renal disease activity, we examined prostaglandin endoperoxide synthetase (PGHS) activity in peripheral-blood monocytes isolated from 5 healthy subjects and 11 untreated patients with biopsy-proven lupus nephritis, using radioimmunoassay of prostaglandin E-2 (PGE(2)) and thromboxane B-2 (TxB(2)) released during 24-hour cultures with selective stimuli/inhibitors. Unstimulated basal PGE(2) and TxB(2) synthesis, reflecting in vivo PGHS activity, was greater in the five patients with active renal involvement (World Health Organization [WHO] classes IVb-c) and the six lupus patients without active disease than in the five healthy subjects (TxB(2), 2,643 +/- 198 [standard error], 2,015 +/- 190, 1,548 +/- 295 pg/10(6) cells, respectively). Escherichia coli lipopolysaccharide (LPS; 10 mu g/mL) potently induced TxB(2) or PGE(2) synthesis in healthy controls (+255% +/- 76% and +611% +/- 190%, +688% +/- 234% and +3,189% +/- 154%; 4 to 24 hours, respectively), an effect abolished by 5 mu mol/L of dexamethasone (DEX) or by 5 mu mol/L of the protein synthesis inhibitor cycloheximide (CHX). Responses to LPS were reduced in lupus patients without disease activity and reduced even further in those with active nephritis. This may be related to substrate depletion or feedback functional inhibition of the inducible isoform of PGHS. Our assay may prove useful in the early detection of kidney disease activity in lupus erythematosus. (C) 1998 by the National Kidney Foundation, Inc.
1998
lupus nephritis; monocytes; prostaglandin e-2; prostaglandin e2; prostaglandin endoperoxide synthetase; systemic lupus erythematosus; thromboxane a(2); thromboxane a2
01 Pubblicazione su rivista::01a Articolo in rivista
Eicosanoid synthesis in peripheral blood monocytes: A marker of disease activity in lupus nephritis / Mene', Paolo; Pecci, Gabriella; Cinotti, Giulio Alberto; Pugliese, Giuseppe; F., Pricci; Pugliese, Francesco. - In: AMERICAN JOURNAL OF KIDNEY DISEASES. - ISSN 0272-6386. - 32:5(1998), pp. 778-784. (Intervento presentato al convegno 29th Annual Meeting of the American-Society-of-Nephrology tenutosi a NEW ORLEANS, LOUISIANA nel NOV 03-06, 1996) [10.1016/s0272-6386(98)70133-7].
File allegati a questo prodotto
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/241752
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 11
  • ???jsp.display-item.citation.isi??? 8
social impact