We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm(-1) and 2800 cm(-1), a "marker band" of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non-destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologies in vivo
Cell apoptosis specific marker by Fourier transform infrarred spectroscopy / Gaudenzi, Silvia; Pozzi, Deleana; Toro, P; Silvestri, Ida; Morrone, Stefania; Congiu, Agostina. - In: SPECTROSCOPY. - ISSN 0887-6703. - STAMPA. - 18:3(2004), pp. 415-422.
Cell apoptosis specific marker by Fourier transform infrarred spectroscopy
GAUDENZI, Silvia;POZZI, Deleana;SILVESTRI, Ida;MORRONE, Stefania;CONGIU, Agostina
2004
Abstract
We used Fourier Transform Infrared Spectroscopy (FTIR) combined with flow cytometry to study the apoptosis and necrosis processes in Jurkat, a lymphocyte cell line. The apoptosis was induced in the cells by a chemical agent, the actinomycin D, while the necrosis was induced lowering the pH value to 4.2. The apoptotic events were analysed by flow cytometry (using annexin V and propidium iodide) and contemporary monitored by FTIR spectroscopy at different times after the treatment. This comparison allowed us to find in the IR spectrum, between 3000 cm(-1) and 2800 cm(-1), a "marker band" of the apoptosis corresponding to the exposure of phosphatidylserine on the outer leaflet of the membrane. A marker of a specific cellular process obtained by using a non-destructive technique such as FTIR spectroscopy, has a great significance in the diagnostic medicine providing a tool for detecting pathologies in vivoI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
