The signalling pathways leading to CXCL8/IL-8-induced human neutrophil migration have not been fully characterized. The present study demonstrates that CXCL8 induces tyrosine phosphorylation as well as enzymatic activity of proline-rich tyrosine kinase 2 (Pyk2), a non-receptor protein tyrosine kinase (PTK), in human neutrophils. Induction of Pyk2 tyrosine phosphorylation by CXCL8 is regulated by Src PTK activation, whereas it is unaffected by phosphatidylinositol 3-kinase activation. Inhibition of Pyk2 activation by PP1, a Src PTK inhibitor, is paralleled by the inhibition of CXCL8-mediated neutrophil chemotaxis. Among CXCL8 receptors, Src protein tyrosine kinase activation selectively regulates CXCR1-mediated polymorphonuclear neutrophil (PMN) chemotaxis. Overexpression of PykM, the kinase-dead mutant of Pyk2, blocks CXCL8-induced chemotaxis of HL-60-derived PMN-like cells, thus pinpointing the key role of Pyk2 in CXCL8-induced chemotaxis.

Key role of proline-rich tyrosine kinase 2 in interleukin-8 (CXCL8/IL-8)-mediated human neutrophil chemotaxis / Vito Di, Cioccio; Strippoli, Raffaele; Cinzia, Bizzarri; Giulia, Troiani; Maria Neve, Cervellera; Isabelle, Gloaguen; Antonella, Colagrande; Elisa Margherita, Cattozzo; Sabrina, Pagliei; Santoni, Angela; Francesco, Colotta; Mainiero, Fabrizio; Riccardo, Bertini. - In: IMMUNOLOGY. - ISSN 0019-2805. - STAMPA. - 111:4(2004), pp. 407-415. [10.1111/j.1365-2567.2004.01822.x]

Key role of proline-rich tyrosine kinase 2 in interleukin-8 (CXCL8/IL-8)-mediated human neutrophil chemotaxis

STRIPPOLI, RAFFAELE;SANTONI, Angela;MAINIERO, Fabrizio;
2004

Abstract

The signalling pathways leading to CXCL8/IL-8-induced human neutrophil migration have not been fully characterized. The present study demonstrates that CXCL8 induces tyrosine phosphorylation as well as enzymatic activity of proline-rich tyrosine kinase 2 (Pyk2), a non-receptor protein tyrosine kinase (PTK), in human neutrophils. Induction of Pyk2 tyrosine phosphorylation by CXCL8 is regulated by Src PTK activation, whereas it is unaffected by phosphatidylinositol 3-kinase activation. Inhibition of Pyk2 activation by PP1, a Src PTK inhibitor, is paralleled by the inhibition of CXCL8-mediated neutrophil chemotaxis. Among CXCL8 receptors, Src protein tyrosine kinase activation selectively regulates CXCR1-mediated polymorphonuclear neutrophil (PMN) chemotaxis. Overexpression of PykM, the kinase-dead mutant of Pyk2, blocks CXCL8-induced chemotaxis of HL-60-derived PMN-like cells, thus pinpointing the key role of Pyk2 in CXCL8-induced chemotaxis.
2004
01 Pubblicazione su rivista::01a Articolo in rivista
Key role of proline-rich tyrosine kinase 2 in interleukin-8 (CXCL8/IL-8)-mediated human neutrophil chemotaxis / Vito Di, Cioccio; Strippoli, Raffaele; Cinzia, Bizzarri; Giulia, Troiani; Maria Neve, Cervellera; Isabelle, Gloaguen; Antonella, Colagrande; Elisa Margherita, Cattozzo; Sabrina, Pagliei; Santoni, Angela; Francesco, Colotta; Mainiero, Fabrizio; Riccardo, Bertini. - In: IMMUNOLOGY. - ISSN 0019-2805. - STAMPA. - 111:4(2004), pp. 407-415. [10.1111/j.1365-2567.2004.01822.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/240398
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