In this work we present a system for the detection of labeled DNA by means of a two-color amorphous silicon photosensor. The device is a p-i-n-i-p structure, whose spectral response is controlled by tuning the voltage applied to its electrodes. The thicknesses of the different layers has been optimized to match the emission spectra of the two utilized fluorochromes. Minima detectable concentrations range in the order of few nmol/l. Very good linearity in the photosensor responses, comparable with those of commercial equipment, has been achieved.
Two-color amorphous silicon photodiode for multicolor detection of labeled DNA / Caputo, Domenico; DE CESARE, Giampiero; Nascetti, Augusto; Negri, Rodolfo; Scipinotti, Riccardo. - 6592:(2007), p. 0C. (Intervento presentato al convegno Microtechnologies for the New Millennium 2007 tenutosi a Maspalomas; Spain nel 02-04 maggio 2007) [10.1117/12.721997].
Two-color amorphous silicon photodiode for multicolor detection of labeled DNA
CAPUTO, Domenico;DE CESARE, Giampiero;NASCETTI, Augusto;NEGRI, RODOLFO;SCIPINOTTI, RICCARDO
2007
Abstract
In this work we present a system for the detection of labeled DNA by means of a two-color amorphous silicon photosensor. The device is a p-i-n-i-p structure, whose spectral response is controlled by tuning the voltage applied to its electrodes. The thicknesses of the different layers has been optimized to match the emission spectra of the two utilized fluorochromes. Minima detectable concentrations range in the order of few nmol/l. Very good linearity in the photosensor responses, comparable with those of commercial equipment, has been achieved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
