The realization of cross talks between transposable elements of class I and their host genome involves non-histonic chromatin proteins. These interactions have been widely analyzed through the characterization of the gypsy retrotransposon leader region, which holds a particularly strong insulator element, and the proteins required for its function, Su(Hw), Mod(mdg4), and Cp190. Here we provide evidence that a similar interaction should occur between ZAM, a gypsy-like element, and HP1, one of the most extensively studied chromatin proteins. We first assayed the existence of this binding using the yeast cells one-hybrid system and then we verified it in vivo by ChIP assay. In order to characterize the interaction between HP1 and the ZAM 5′ untranslated region we performed a series of gel shift analyses. Our observations confirm an HP1 co-operative DNA-binding and display for the first time the HP1 DNA target motif that, we hypothesize, should be one of its nucleation sites. © 2007 Elsevier B.V. All rights reserved.
Heterochromatin protein 1 interacts with 5′UTR of transposable element ZAM in a sequence-specific fashion / Crescenzio Francesco, Minervini; Rene' Massimiliano, Marsano; Paola, Casieri; Fanti, Laura; Ruggiero, Caizzi; Pimpinelli, Sergio; Mariano, Rocchi; Luigi, Viggiano. - In: GENE. - ISSN 0378-1119. - 393:1-2(2007), pp. 1-10. [10.1016/j.gene.2006.12.028]
Heterochromatin protein 1 interacts with 5′UTR of transposable element ZAM in a sequence-specific fashion
FANTI, Laura;PIMPINELLI, Sergio;
2007
Abstract
The realization of cross talks between transposable elements of class I and their host genome involves non-histonic chromatin proteins. These interactions have been widely analyzed through the characterization of the gypsy retrotransposon leader region, which holds a particularly strong insulator element, and the proteins required for its function, Su(Hw), Mod(mdg4), and Cp190. Here we provide evidence that a similar interaction should occur between ZAM, a gypsy-like element, and HP1, one of the most extensively studied chromatin proteins. We first assayed the existence of this binding using the yeast cells one-hybrid system and then we verified it in vivo by ChIP assay. In order to characterize the interaction between HP1 and the ZAM 5′ untranslated region we performed a series of gel shift analyses. Our observations confirm an HP1 co-operative DNA-binding and display for the first time the HP1 DNA target motif that, we hypothesize, should be one of its nucleation sites. © 2007 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
