Stanozolol is an anabolic steroid illicitly used for growth promoting purposes in animal prodn. For reasons of public health the use of anabolic steroids as growth promoters is officially banned in Europe in animals intended for consumption. The aim of this work is the investigation of stanozolol metabolites, 3'-hydroxystanozolol, 4.beta.-hydroxystanozolol and 16.beta.-hydroxystanozolol in urine samples after stanozolol administration to a young calf. After solid phase extn. of urine samples, detection is carried out by HPLC-MS-MS multiple reaction monitoring. Av. recovery for the three metabolites is 80%. The method is highly specific and has been validated in terms of linearity, inter and intra day precision. In addn. the decision limit CC.alpha. and the detection capability CC.beta. have been detected
Excretion study of stanozolol in bovine by HPLC-tandem mass spectrometry / Buiarelli, Francesca; G. P., Cartoni; Coccioli, Franco; M., Merolle; B., Neri. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 60:9-10(2004), pp. 545-551. [10.1365/s10337-004-0400-z]
Excretion study of stanozolol in bovine by HPLC-tandem mass spectrometry
BUIARELLI, Francesca;COCCIOLI, Franco;
2004
Abstract
Stanozolol is an anabolic steroid illicitly used for growth promoting purposes in animal prodn. For reasons of public health the use of anabolic steroids as growth promoters is officially banned in Europe in animals intended for consumption. The aim of this work is the investigation of stanozolol metabolites, 3'-hydroxystanozolol, 4.beta.-hydroxystanozolol and 16.beta.-hydroxystanozolol in urine samples after stanozolol administration to a young calf. After solid phase extn. of urine samples, detection is carried out by HPLC-MS-MS multiple reaction monitoring. Av. recovery for the three metabolites is 80%. The method is highly specific and has been validated in terms of linearity, inter and intra day precision. In addn. the decision limit CC.alpha. and the detection capability CC.beta. have been detectedI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
