We have identified, expressed and characterized two genes from Arabidopsis thaliana (AtPMEI-1 and AtPMEI-2) encoding functional inhibitors of pectin methylesterases. AtPMEI-1 and AtPMEI-2 are cell wall proteins sharing many features with the only pectin methylesterase inhibitor (PMEI) characterized so far from kiwi fruit. Both Arabidopsis proteins interact with and inhibit plant-derived pectin methylesterases (PMEs) but not microbial enzymes. The occurrence of functional PMEIs in Arabidopsis indicates that a mechanism of controlling pectin esterification by inhibition of endogenous PMEs is present in different plant species. © 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors / A., Raiola; L., Camardella; A., Giovane; Mattei, Maria Benedetta; DE LORENZO, Giulia; Cervone, Felice; Bellincampi, Daniela. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 557:1-3(2004), pp. 199-203. [10.1016/s0014-5793(03)01491-1]
Two Arabidopsis thaliana genes encode functional pectin methylesterase inhibitors
MATTEI, Maria Benedetta;DE LORENZO, Giulia;CERVONE, Felice;BELLINCAMPI, Daniela
2004
Abstract
We have identified, expressed and characterized two genes from Arabidopsis thaliana (AtPMEI-1 and AtPMEI-2) encoding functional inhibitors of pectin methylesterases. AtPMEI-1 and AtPMEI-2 are cell wall proteins sharing many features with the only pectin methylesterase inhibitor (PMEI) characterized so far from kiwi fruit. Both Arabidopsis proteins interact with and inhibit plant-derived pectin methylesterases (PMEs) but not microbial enzymes. The occurrence of functional PMEIs in Arabidopsis indicates that a mechanism of controlling pectin esterification by inhibition of endogenous PMEs is present in different plant species. © 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
