We previously reported that Saccharomyces cerevisiae mutants in mRNA decapping and mutants expressing a truncated form of the KILSM4 gene, showed premature senescence and apoptotic phenotypes. Here, we show that this truncated protein is dispersed in the cytoplasm and does not assemble to P-bodies. As reported in decapping mutants, we observed an increase in the number of P-bodies suggesting that the truncation of the protein impairs this process. The number of P-bodies also increases after oxidative stress and is not dependent on the meta-caspase gene YCA1, placing this phenomenon upstream to the onset of apoptosis. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
The C-terminus of the yeast Lsm4p is required for the association to P-bodies / Mazzoni, Cristina; I., Daddario; Falcone, Claudio. - In: FEBS LETTERS. - ISSN 0014-5793. - 581:25(2007), pp. 4836-4840. [10.1016/j.febslet.2007.09.009]
The C-terminus of the yeast Lsm4p is required for the association to P-bodies
MAZZONI, Cristina;FALCONE, Claudio
2007
Abstract
We previously reported that Saccharomyces cerevisiae mutants in mRNA decapping and mutants expressing a truncated form of the KILSM4 gene, showed premature senescence and apoptotic phenotypes. Here, we show that this truncated protein is dispersed in the cytoplasm and does not assemble to P-bodies. As reported in decapping mutants, we observed an increase in the number of P-bodies suggesting that the truncation of the protein impairs this process. The number of P-bodies also increases after oxidative stress and is not dependent on the meta-caspase gene YCA1, placing this phenomenon upstream to the onset of apoptosis. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
