We previously reported that during death receptor-mediated apoptosis, cardiolipin (CL) relocates to the cell surface, where it reacts with autoantibodies from antiphospholipid syndrome sera. Here, we analysed the intracellular distribution of CL and its metabolites during the early phase of cell death signalling triggered by Fas stimulation in U937 cells and mouse liver. We found a redistribution of mitochondrial CL to the cell surface by using confocal microscopy and flow cytometry. Mass spectrometry revealed that CL and its metabolites relocated from mitochondria to other intracellular organelles during apoptosis, with a conversion into non-mitochondrial lipids. Concomitantly, cytosolic Bid relocated to the light membranes comprised in fraction P100, including the plasma membrane and associated vesicular systems. A direct Bid-CL interaction was demonstrated by the observation that CL and monolysoCL coimmunoprecipitated with Bid especially after Fas stimulation, suggesting a dynamic interaction of the protein with CL and its metabolites. © 2004 Nature Publishing Group All rights reserved.

Cardiolipin and its metabolites move from mitochondria to other cellular membranes during death receptor-mediated apoptosis / Sorice, Maurizio; A., Circella; I. M., Cristea; Garofalo, Tina; DI RENZO, Livia Maria; Alessandri, Cristiano; Valesini, Guido; M., Degli Esposti. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 11:10(2004), pp. 1133-1145. [10.1038/sj.cdd.4401457]

Cardiolipin and its metabolites move from mitochondria to other cellular membranes during death receptor-mediated apoptosis

SORICE, Maurizio;GAROFALO, TINA;DI RENZO, Livia Maria;ALESSANDRI, cristiano;VALESINI, Guido;
2004

Abstract

We previously reported that during death receptor-mediated apoptosis, cardiolipin (CL) relocates to the cell surface, where it reacts with autoantibodies from antiphospholipid syndrome sera. Here, we analysed the intracellular distribution of CL and its metabolites during the early phase of cell death signalling triggered by Fas stimulation in U937 cells and mouse liver. We found a redistribution of mitochondrial CL to the cell surface by using confocal microscopy and flow cytometry. Mass spectrometry revealed that CL and its metabolites relocated from mitochondria to other intracellular organelles during apoptosis, with a conversion into non-mitochondrial lipids. Concomitantly, cytosolic Bid relocated to the light membranes comprised in fraction P100, including the plasma membrane and associated vesicular systems. A direct Bid-CL interaction was demonstrated by the observation that CL and monolysoCL coimmunoprecipitated with Bid especially after Fas stimulation, suggesting a dynamic interaction of the protein with CL and its metabolites. © 2004 Nature Publishing Group All rights reserved.
2004
bid; cardiolipin; mitochondria; phospholipids
01 Pubblicazione su rivista::01a Articolo in rivista
Cardiolipin and its metabolites move from mitochondria to other cellular membranes during death receptor-mediated apoptosis / Sorice, Maurizio; A., Circella; I. M., Cristea; Garofalo, Tina; DI RENZO, Livia Maria; Alessandri, Cristiano; Valesini, Guido; M., Degli Esposti. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 11:10(2004), pp. 1133-1145. [10.1038/sj.cdd.4401457]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/236466
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