Immobilization of mushroom tyrosinase on controlled pore glass: Effect of chemical modification

Mushroom tyrosinase immobilization on controlled pore glass with three different functional groups like alkylamino, carboxyl and arylamino is performed. The effect of the alkylamino-CPG arm extension on enzymatic stability and catalytic activity is also investigated. The results expressed in terms of ligand coupling, % yield and kinetic parameters, like the apparent K-m(app) and V-max(app) and the inherent K-m(inh) and V-max(inh) values demonstrate the best performance of tyrosinase immobilized on alkylammino-CPGs. (c) 2007 Elsevier B.V All rights reserved.