Aim: To reassess the cut-off value for lamellar body counts (LBs) for fetal lung maturity (FLM) over a 10-year study period. Patients and methods: 178 pregnancies were selected under strict inclusion criteria and delivered within 48 h from amniocentesis. FLM was determined by amniotic fluid LBs in centrifuged samples (300 × g for 10 min) in a commercially available Coulter Counter. Cases beyond 37 weeks were excluded. Results: Mean gestational age was 33.5±3.0 weeks at amniocentesis and 33.7±3.0 weeks at birth. After reassessing the best compromise between sensitivity and specificity for all cases using the receiver operating characteristic (ROC) procedure, an FLM cut-off value of ≤22,000/μL was obtained. Diagnostic accuracy (and confidence interval, CI) was: sensitivity, 73% (60.0-83.6%); specificity, 81.7% (CI 73.6-88.1%); positive predictive value, 66.2%; and negative predictive value, 86.0%. Conclusion: No significant change in FLM cut-off for LBs was found when comparing the value from this study and the results of our earlier report presented in 1996 (≤22,000 vs. <20,000/μL), although the new value may be more accurate, since it is based on neonatal outcome, with the exclusion of cases in which the diagnosis of FLM is seldom warranted, i.e., ≥37 weeks' gestational age.
Amniotic fluid lamellar body counts for the determination of fetal lung maturity: An update / Juan J., Piazze; Luca, Maranghi; Albana, Cerekja; Paolo, Meloni; Stefano, Gioia; Luisa, Fumian; Cosmi, Ermelando; Anceschi, Maurizio Marco. - In: JOURNAL OF PERINATAL MEDICINE. - ISSN 0300-5577. - 33:2(2005), pp. 156-160. [10.1515/jpm.2005.029]
Amniotic fluid lamellar body counts for the determination of fetal lung maturity: An update
COSMI, Ermelando;ANCESCHI, Maurizio Marco
2005
Abstract
Aim: To reassess the cut-off value for lamellar body counts (LBs) for fetal lung maturity (FLM) over a 10-year study period. Patients and methods: 178 pregnancies were selected under strict inclusion criteria and delivered within 48 h from amniocentesis. FLM was determined by amniotic fluid LBs in centrifuged samples (300 × g for 10 min) in a commercially available Coulter Counter. Cases beyond 37 weeks were excluded. Results: Mean gestational age was 33.5±3.0 weeks at amniocentesis and 33.7±3.0 weeks at birth. After reassessing the best compromise between sensitivity and specificity for all cases using the receiver operating characteristic (ROC) procedure, an FLM cut-off value of ≤22,000/μL was obtained. Diagnostic accuracy (and confidence interval, CI) was: sensitivity, 73% (60.0-83.6%); specificity, 81.7% (CI 73.6-88.1%); positive predictive value, 66.2%; and negative predictive value, 86.0%. Conclusion: No significant change in FLM cut-off for LBs was found when comparing the value from this study and the results of our earlier report presented in 1996 (≤22,000 vs. <20,000/μL), although the new value may be more accurate, since it is based on neonatal outcome, with the exclusion of cases in which the diagnosis of FLM is seldom warranted, i.e., ≥37 weeks' gestational age.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.