TPA, a potent PKC activator, inhibits myogenic differentiation and activates phospholipase D (PLD). We evaluated the involvement of PLD in the TPA effects on L6 myoblasts differentiation. TPA, at concentrations inhibiting differentiation of L6 cells, induced a strong, though transient, PLD activation. Surprisingly, at nanomolar concentration, TPA induced both myogenic differentiation and sustained activation of PLD. Differential effect of TPA can be ascribed to PKC downregulation induced by highest TPA concentrations. TPA-induced differentiation was inhibited by 1-butanol, confirming the involvement of PLD in this effect. These data suggest that prolonged elevation of PLD activity is required for myogenic differentiation. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Phorbol ester-induced differentiation of L6 myogenic cells involves phospholipase D activation / Hiba, Komati; Alessandra, Minasi; Naro, Fabio; Michel, Lagarde; Annie France, Prigent; Adamo, Sergio; Georges, Nemoz. - In: FEBS LETTERS. - ISSN 0014-5793. - STAMPA. - 577:3(2004), pp. 409-414. [10.1016/j.febslet.2004.10.036]
Phorbol ester-induced differentiation of L6 myogenic cells involves phospholipase D activation
NARO, Fabio;ADAMO, Sergio;
2004
Abstract
TPA, a potent PKC activator, inhibits myogenic differentiation and activates phospholipase D (PLD). We evaluated the involvement of PLD in the TPA effects on L6 myoblasts differentiation. TPA, at concentrations inhibiting differentiation of L6 cells, induced a strong, though transient, PLD activation. Surprisingly, at nanomolar concentration, TPA induced both myogenic differentiation and sustained activation of PLD. Differential effect of TPA can be ascribed to PKC downregulation induced by highest TPA concentrations. TPA-induced differentiation was inhibited by 1-butanol, confirming the involvement of PLD in this effect. These data suggest that prolonged elevation of PLD activity is required for myogenic differentiation. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
