The reactive oxygen species (ROS) produced inside cells by UV-B radiation may induce apoptosis, a process that realizes the programmed death of cells. In the present paper the UV induced damage was studied in a human T-Lymphocyte cell line (the Jurkat line) by mean of IR laser photoacoustic spectroscopy (PAS) and by Fourier Transform Infrared Spectroscopy (FTIR) combined with biological assays based on flow cytometry. The apoptosis was induced in vitro in the Jurkat samples by exposition to UV-B radiation with a dose of 310 mJ/cm2. PAS measurements were performed through a 10W c.w. CO2 laser based optical system realised at ENEA Molecular Spectroscopy Laboratory in Frascati (Italy).
UV-B radiation induced effects on human T-lymphocytes / G., Giubileo; Congiu, Agostina; Gaudenzi, Silvia; P., Grimaldi; Pozzi, Deleana. - 6785:(2007), pp. 67851H-1-67851H-10. (Intervento presentato al convegno ROMOPTO 2006: Eighth Conference on Optics tenutosi a Sibiu; Romania nel 4 September 2006 through 7 September 2006) [10.1117/12.757866].
UV-B radiation induced effects on human T-lymphocytes
CONGIU, Agostina;GAUDENZI, Silvia;POZZI, Deleana
2007
Abstract
The reactive oxygen species (ROS) produced inside cells by UV-B radiation may induce apoptosis, a process that realizes the programmed death of cells. In the present paper the UV induced damage was studied in a human T-Lymphocyte cell line (the Jurkat line) by mean of IR laser photoacoustic spectroscopy (PAS) and by Fourier Transform Infrared Spectroscopy (FTIR) combined with biological assays based on flow cytometry. The apoptosis was induced in vitro in the Jurkat samples by exposition to UV-B radiation with a dose of 310 mJ/cm2. PAS measurements were performed through a 10W c.w. CO2 laser based optical system realised at ENEA Molecular Spectroscopy Laboratory in Frascati (Italy).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
