The methanol extract from Hypericum hircinum leaves exhibited in vitro inhibition of monoamine oxidases (MAO). Bioassay-guided fractionation led to the isolation of quercetin and five compounds identified for the first time from H. hircinum. Quercetin was the only compound with a selective inhibitory activity against MAO-A, with an IC50 value of 0.010 mu M. To explain MAO selective inhibition at the molecular level, a computational study was carried out by conformational search and docking techniques using recently determined crystallographic models of both enzymatic isoforms. An in vivo study in mice was carried out using the forced swimming test in order to elucidate the behavioral effects of quercetin.
Quercetin as the active principle of Hypericum hircinum exerts a selective inhibitory activity against MAO-A: Extraction, biological analysis, and computational study / F., Chimenti; F., Cottiglia; L., Bonsignore; L., Casu; M., Casu; C., Floris; Secci, Daniela; Bolasco, Adriana; Chimenti, Paola; Granese, Arianna; O., Befani; Turini, Paola; S., Alcaro; F., Ortuso; G., Trombetta; A., Loizzo; I., Guarino. - In: JOURNAL OF NATURAL PRODUCTS. - ISSN 0163-3864. - 69:6(2006), pp. 945-949. [10.1021/np060015w]
Quercetin as the active principle of Hypericum hircinum exerts a selective inhibitory activity against MAO-A: Extraction, biological analysis, and computational study
SECCI, DANIELA;BOLASCO, Adriana;CHIMENTI, Paola;GRANESE, ARIANNA;TURINI, Paola;
2006
Abstract
The methanol extract from Hypericum hircinum leaves exhibited in vitro inhibition of monoamine oxidases (MAO). Bioassay-guided fractionation led to the isolation of quercetin and five compounds identified for the first time from H. hircinum. Quercetin was the only compound with a selective inhibitory activity against MAO-A, with an IC50 value of 0.010 mu M. To explain MAO selective inhibition at the molecular level, a computational study was carried out by conformational search and docking techniques using recently determined crystallographic models of both enzymatic isoforms. An in vivo study in mice was carried out using the forced swimming test in order to elucidate the behavioral effects of quercetin.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
