Thrombin, its main inhibitor (protease nexin-1) and its related receptors (protease-activated receptors, PAR-1,-2, -3, -4) were studied in rat hippocampus following administration of trimethyltin (TMT), a neurotoxin inducing neuronal degeneration and reactive gliosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry revealed that while expression of prothrombin and protease nexin-1 did not change significantly in TMT-treated hippocampi, PARs (in particular PAR-1 and to a lesser extent PAR-2 and PAR-3) were upregulated in reactive astrocytes, suggesting their involvement in neurodegeneration and in the consequent response of the nervous tissue.
Trimethyltin-induced differential expression of PAR subtypes in reactive astrocytes of the rat hippocampus / Pompili, Elena; Stefania Lucia, Nori; Maria Concetta, Geloso; Enrico, Guadagni; Valentina, Corvino; Fabrizio, Michetti; Fumagalli, Lorenzo. - In: MOLECULAR BRAIN RESEARCH. - ISSN 0169-328X. - 122:1(2004), pp. 93-98. [10.1016/j.molbrainres.2003.12.001]
Trimethyltin-induced differential expression of PAR subtypes in reactive astrocytes of the rat hippocampus
POMPILI, Elena;FUMAGALLI, Lorenzo
2004
Abstract
Thrombin, its main inhibitor (protease nexin-1) and its related receptors (protease-activated receptors, PAR-1,-2, -3, -4) were studied in rat hippocampus following administration of trimethyltin (TMT), a neurotoxin inducing neuronal degeneration and reactive gliosis. Reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry revealed that while expression of prothrombin and protease nexin-1 did not change significantly in TMT-treated hippocampi, PARs (in particular PAR-1 and to a lesser extent PAR-2 and PAR-3) were upregulated in reactive astrocytes, suggesting their involvement in neurodegeneration and in the consequent response of the nervous tissue.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
