Images of terrestrial and marine invertebrates (snails and bivalves) have been obtained by using an X-ray phase-contrast imaging technique, namely, synchrotron-based diffraction-enhanced imaging. Synchrotron X-rays of 20, 30 and 40 key were used, which penetrate deep enough into animal soft tissues. The phase of X-ray photons shifts slightly as they traverse an object, such as animal soft tissue, and interact with its atoms. Biological features, such as shell morphology and animal physiology, have been visualized. The contrast of the images obtained at 40 key is the best. This optimum energy provided a clear view of the internal structural organization of the soft tissue with better contrast. The contrast is higher at edges of internal soft-tissue structures. The image improvements achieved with the diffraction-enhanced imaging technique are due to extinction, i.e., elimination of ultra-small-angle scattering. They enabled us to identify a few embedded internal shell features, such as the origin of the apex, which is the firmly attached region of the soft tissue connecting the umbilicus to the external morphology. Diffraction-enhanced imaging can provide high-quality images of soft tissues valuable for biology. (C) 2010 Elsevier Ltd. All rights reserved.

Use of synchrotron-based diffraction-enhanced imaging for visualization of soft tissues in invertebrates / Donepudi V., Rao; M., Cesareo Swapna; Antonio, Brunetti; Zhong, Zhong; Takao, Akatsuka; Tohoru, Takeda; Gigante, Giovanni Ettore. - In: APPLIED RADIATION AND ISOTOPES. - ISSN 0969-8043. - 68:9(2010), pp. 1687-1693. [10.1016/j.apradiso.2010.04.001]

Use of synchrotron-based diffraction-enhanced imaging for visualization of soft tissues in invertebrates

GIGANTE, Giovanni Ettore
2010

Abstract

Images of terrestrial and marine invertebrates (snails and bivalves) have been obtained by using an X-ray phase-contrast imaging technique, namely, synchrotron-based diffraction-enhanced imaging. Synchrotron X-rays of 20, 30 and 40 key were used, which penetrate deep enough into animal soft tissues. The phase of X-ray photons shifts slightly as they traverse an object, such as animal soft tissue, and interact with its atoms. Biological features, such as shell morphology and animal physiology, have been visualized. The contrast of the images obtained at 40 key is the best. This optimum energy provided a clear view of the internal structural organization of the soft tissue with better contrast. The contrast is higher at edges of internal soft-tissue structures. The image improvements achieved with the diffraction-enhanced imaging technique are due to extinction, i.e., elimination of ultra-small-angle scattering. They enabled us to identify a few embedded internal shell features, such as the origin of the apex, which is the firmly attached region of the soft tissue connecting the umbilicus to the external morphology. Diffraction-enhanced imaging can provide high-quality images of soft tissues valuable for biology. (C) 2010 Elsevier Ltd. All rights reserved.
2010
diffraction-enhanced imaging; invertebrates; soft tissue; synchrotron
01 Pubblicazione su rivista::01a Articolo in rivista
Use of synchrotron-based diffraction-enhanced imaging for visualization of soft tissues in invertebrates / Donepudi V., Rao; M., Cesareo Swapna; Antonio, Brunetti; Zhong, Zhong; Takao, Akatsuka; Tohoru, Takeda; Gigante, Giovanni Ettore. - In: APPLIED RADIATION AND ISOTOPES. - ISSN 0969-8043. - 68:9(2010), pp. 1687-1693. [10.1016/j.apradiso.2010.04.001]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/23161
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