In this work we present, for the first time, a rapid, compact and innovative method for detection of Ochratoxin A ( OTA) based on hydrogenated amorphous silicon (a-Si: H) sensors. 2 mu l of acidified toluene containing OTA at different concentrations were spotted on the silica side of a High Performance Thin Layer Cromatography plate and aligned with a a-Si: H p- i- n photodiode deposited by Plasma Enhanced Chemical Vapor Deposition on a different glass substrate. As an UV radiation excites the mycotoxin, the re-emitted light is detected by the a- Si: H sensor. Results show a very good linearity between OTA concentration and the sensor photocurrent over almost three orders of magnitude. The minimum detected OTA concentration is equal to 0.1ng, showing that the presented system has the potential for a low cost system suitable for the early detection of toxins in foods.
Innovative detection system of ochratoxin a by thin film photodiodes / Caputo, Domenico; DE CESARE, Giampiero; Fanelli, Corrado; Nascetti, Augusto; Alessandra, Ricelli; Scipinotti, Riccardo. - In: SENSORS. - ISSN 1424-8220. - 7:7(2007), pp. 1317-1322. [10.3390/s7071317]
Innovative detection system of ochratoxin a by thin film photodiodes
CAPUTO, Domenico;DE CESARE, Giampiero;FANELLI, Corrado;NASCETTI, Augusto;SCIPINOTTI, RICCARDO
2007
Abstract
In this work we present, for the first time, a rapid, compact and innovative method for detection of Ochratoxin A ( OTA) based on hydrogenated amorphous silicon (a-Si: H) sensors. 2 mu l of acidified toluene containing OTA at different concentrations were spotted on the silica side of a High Performance Thin Layer Cromatography plate and aligned with a a-Si: H p- i- n photodiode deposited by Plasma Enhanced Chemical Vapor Deposition on a different glass substrate. As an UV radiation excites the mycotoxin, the re-emitted light is detected by the a- Si: H sensor. Results show a very good linearity between OTA concentration and the sensor photocurrent over almost three orders of magnitude. The minimum detected OTA concentration is equal to 0.1ng, showing that the presented system has the potential for a low cost system suitable for the early detection of toxins in foods.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
