The expression of the extracellular matrix-related genes, such as fibronectin, laminin and tenascin C, and apoptosis-related genes, such as bax, bcl2 and survivin, was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and by immunohistochemistry in normal breast tissue and benign and malignant breast tumors and then correlated to several clinical parameters: estrogen and progesterone receptors, Ki67, ErbB2, tumor size, lymph node status and grading. Seventy-three breast tissue samples were examined. After RNA extraction, an RT-PCR was performed to detect fibronectin, laminin, tenascin C, bax, bcl2 and survivin gene expression. Thirty-two samples were evaluated also by immunohistochemistry at the protein level to detect fibronectin, laminin, tenascin C, bax and survivin. We found a significant correlation (P=0.025) between fibronectin gene expression and lymph node status, and a significant negative correlation (P=0.049) between laminin gene expression and Ki67. In addition, we found a statistically significant increase in survivin transcription in malign tumors compared to fibroadenomas (P=0.024). The negative correlation between laminin transcription and Ki67 could suggest that laminin impacts negatively on tumor proliferation, and the positive correlation between fibronectin and lymph node status may lead to consider fibronectin as predictive of long distance metastasis.

Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast / Fortunata, Vasaturo; E., Sallusti; Gradilone, Angela; C., Malacrino; T., Nardo; G., Avagnano; Agliano', Anna Maria; T., Granato; B., Vincenzi; G., Coppotelli; A., Marzullo; Soda, Giuseppe; Simonelli, Lucilla; Modesti, Mauro; Scarpa, Susanna. - In: INTERNATIONAL JOURNAL OF ONCOLOGY. - ISSN 1019-6439. - STAMPA. - 27:4(2005), pp. 1005-1011.

Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast

GRADILONE, Angela;AGLIANO', Anna Maria;SODA, Giuseppe;SIMONELLI, Lucilla;MODESTI, Mauro;SCARPA, Susanna
2005

Abstract

The expression of the extracellular matrix-related genes, such as fibronectin, laminin and tenascin C, and apoptosis-related genes, such as bax, bcl2 and survivin, was evaluated by reverse transcription-polymerase chain reaction (RT-PCR) and by immunohistochemistry in normal breast tissue and benign and malignant breast tumors and then correlated to several clinical parameters: estrogen and progesterone receptors, Ki67, ErbB2, tumor size, lymph node status and grading. Seventy-three breast tissue samples were examined. After RNA extraction, an RT-PCR was performed to detect fibronectin, laminin, tenascin C, bax, bcl2 and survivin gene expression. Thirty-two samples were evaluated also by immunohistochemistry at the protein level to detect fibronectin, laminin, tenascin C, bax and survivin. We found a significant correlation (P=0.025) between fibronectin gene expression and lymph node status, and a significant negative correlation (P=0.049) between laminin gene expression and Ki67. In addition, we found a statistically significant increase in survivin transcription in malign tumors compared to fibroadenomas (P=0.024). The negative correlation between laminin transcription and Ki67 could suggest that laminin impacts negatively on tumor proliferation, and the positive correlation between fibronectin and lymph node status may lead to consider fibronectin as predictive of long distance metastasis.
2005
apoptosis; breast cancer; extracellular matrix
01 Pubblicazione su rivista::01a Articolo in rivista
Comparison of extracellular matrix and apoptotic markers between benign lesions and carcinomas in human breast / Fortunata, Vasaturo; E., Sallusti; Gradilone, Angela; C., Malacrino; T., Nardo; G., Avagnano; Agliano', Anna Maria; T., Granato; B., Vincenzi; G., Coppotelli; A., Marzullo; Soda, Giuseppe; Simonelli, Lucilla; Modesti, Mauro; Scarpa, Susanna. - In: INTERNATIONAL JOURNAL OF ONCOLOGY. - ISSN 1019-6439. - STAMPA. - 27:4(2005), pp. 1005-1011.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/231175
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