An original synthetic methodwas developed for the preparation of a family of six novel deactivated restricted-access materials (RAMs), belonging to the group of the internal surface reversed-phase (ISRP) supports. The supports (ISRP-RAM phases A–F) have an alkyl-chain (14 methylenes) with two embedded ureido groups bound only to the internal surfaces of the porous silica, and polyvinyl alcoholic groups (PVA, 100 000→22 000 molecular weight) chemically bound to the external surfaces. The average pore diameters of the prepared ISRP-RAM supports, calculated by inverse size-exclusion chromatography, ranged between 49 °A and 88A° , and were able to exclude macromolecules heavier than about 24 000Da (such as serum proteins) from the pores. The novel supports were designed for the determination of a semi-synthetic anticance
Synthesis and characterization of novel internal surface reversed-phase silica supports for high-performance liquid chromatography / Gasparrini, Francesco; Ciogli, Alessia; D'Acquarica, Ilaria; Misiti, Domenico; Elena, Badaloni; Fabrizio, Giorgi; Aristide, Vigevani. - In: JOURNAL OF CHROMATOGRAPHY A. - ISSN 0021-9673. - 1176:1-2(2007), pp. 79-88. [10.1016/j.chroma.2007.10.062]
Synthesis and characterization of novel internal surface reversed-phase silica supports for high-performance liquid chromatography
GASPARRINI, Francesco;CIOGLI, Alessia;D'ACQUARICA, Ilaria;MISITI, Domenico;
2007
Abstract
An original synthetic methodwas developed for the preparation of a family of six novel deactivated restricted-access materials (RAMs), belonging to the group of the internal surface reversed-phase (ISRP) supports. The supports (ISRP-RAM phases A–F) have an alkyl-chain (14 methylenes) with two embedded ureido groups bound only to the internal surfaces of the porous silica, and polyvinyl alcoholic groups (PVA, 100 000→22 000 molecular weight) chemically bound to the external surfaces. The average pore diameters of the prepared ISRP-RAM supports, calculated by inverse size-exclusion chromatography, ranged between 49 °A and 88A° , and were able to exclude macromolecules heavier than about 24 000Da (such as serum proteins) from the pores. The novel supports were designed for the determination of a semi-synthetic anticanceI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
