SDS-CTAB cat-anionic vesicles are supramolecular aggregates forming complexes with biopolymers and enter the cells via membrane fusion or endocytosis. Different applicative areas exist: gene therapy, drug delivery and nanotechnology. We previously examined the absorption/release of biopolymers from vesicles in solution. Here we evaluate their cytotoxicity in cultured cells; to this end we characterized the vesicles and analyzed their biological effects at cellular and molecular level. At low concentration these vesicles have scarce consequences on normal cell growth; at higher dosage they activate apoptotic death processes, due to membrane damage. In conclusion, the use of these particles in nano-biotechnology represents an actual possibility. (C) 2010 Elsevier B.V. All rights reserved.
Biological activity of SDS-CTAB cat-anionic vesicles in cultured cells and assessment of their cytotoxicity ending in apoptosis / Cecilia, Aiello; Andreozzi, Patrizia; LA MESA, Camillo; Risuleo, Gianfranco. - In: COLLOIDS AND SURFACES. B, BIOINTERFACES. - ISSN 0927-7765. - STAMPA. - 78:2(2010), pp. 149-154. [10.1016/j.colsurfb.2010.02.013]
Biological activity of SDS-CTAB cat-anionic vesicles in cultured cells and assessment of their cytotoxicity ending in apoptosis
ANDREOZZI, PATRIZIA;LA MESA, Camillo;RISULEO, Gianfranco
2010
Abstract
SDS-CTAB cat-anionic vesicles are supramolecular aggregates forming complexes with biopolymers and enter the cells via membrane fusion or endocytosis. Different applicative areas exist: gene therapy, drug delivery and nanotechnology. We previously examined the absorption/release of biopolymers from vesicles in solution. Here we evaluate their cytotoxicity in cultured cells; to this end we characterized the vesicles and analyzed their biological effects at cellular and molecular level. At low concentration these vesicles have scarce consequences on normal cell growth; at higher dosage they activate apoptotic death processes, due to membrane damage. In conclusion, the use of these particles in nano-biotechnology represents an actual possibility. (C) 2010 Elsevier B.V. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
