The Aurora kinases regulate chromosome segregation and cytokinesis, and alterations in their expression associate with cell malignant transformation. In this study, we demonstrated by qRT-PCR analysis of 14 seminomas that Aurora-A mRNA was, with respect to control tissues, augmented in five of 14 tumour tissues by 2.17 ± 0.30 fold (P < 0.05) and reduced in 9 to 0.38 ± 0.10 (P < 0.01). Aurora-B mRNA was increased in 11 tumour tissues by 4.33 ± 0.82 fold (P < 0.01) and reduced in 3 to 0.41 ± 0.11 fold. Aurora-C mRNA was reduced to 0.20 ± 0.32 fold (P < 0.01) in 13 seminomas and up-regulated in one case. Western blot experiments, performed on protein extracts of nine seminomas and six normal testes, showed an up-regulation of Aurora-B protein by 10.14 ± 3.51 fold (P < 0.05), while Aurora-A protein was found increased in four seminomas by 2.16 ± 0.43 (P < 0.05), unchanged in three and reduced in two tumour tissues. Aurora-C protein was increased by 9.2 ± 2.90 fold (P < 0.05), suggesting that post-transcriptional mechanisms modulate its expression. In conclusion, we demonstrated that expression of Aurora kinases is deregulated in seminomas, suggesting that they may play a role in the progression of testicular cancers. © 2010 Blackwell Verlag GmbH.

Deregulation of Aurora kinase gene expression in human testicular germ cell tumours / Baldini, Enke; Y., Arlot Bonnemains; M., Mottolese; S., Sentinelli; B., Antoniani; Sorrenti, Salvatore; Salducci, Mauro; E., Comini; Ulisse, Salvatore; D'Armiento, Massimino. - In: ANDROLOGIA. - ISSN 0303-4569. - 42:4(2010), pp. 260-267. [10.1111/j.1439-0272.2009.00987.x]

Deregulation of Aurora kinase gene expression in human testicular germ cell tumours

BALDINI, ENKE;SORRENTI, Salvatore;SALDUCCI, Mauro;ULISSE, SALVATORE;D'ARMIENTO, Massimino
2010

Abstract

The Aurora kinases regulate chromosome segregation and cytokinesis, and alterations in their expression associate with cell malignant transformation. In this study, we demonstrated by qRT-PCR analysis of 14 seminomas that Aurora-A mRNA was, with respect to control tissues, augmented in five of 14 tumour tissues by 2.17 ± 0.30 fold (P < 0.05) and reduced in 9 to 0.38 ± 0.10 (P < 0.01). Aurora-B mRNA was increased in 11 tumour tissues by 4.33 ± 0.82 fold (P < 0.01) and reduced in 3 to 0.41 ± 0.11 fold. Aurora-C mRNA was reduced to 0.20 ± 0.32 fold (P < 0.01) in 13 seminomas and up-regulated in one case. Western blot experiments, performed on protein extracts of nine seminomas and six normal testes, showed an up-regulation of Aurora-B protein by 10.14 ± 3.51 fold (P < 0.05), while Aurora-A protein was found increased in four seminomas by 2.16 ± 0.43 (P < 0.05), unchanged in three and reduced in two tumour tissues. Aurora-C protein was increased by 9.2 ± 2.90 fold (P < 0.05), suggesting that post-transcriptional mechanisms modulate its expression. In conclusion, we demonstrated that expression of Aurora kinases is deregulated in seminomas, suggesting that they may play a role in the progression of testicular cancers. © 2010 Blackwell Verlag GmbH.
2010
aneuploidy; aurora kinases; genomic instability; mitosis; testicular germ cell tumour
01 Pubblicazione su rivista::01a Articolo in rivista
Deregulation of Aurora kinase gene expression in human testicular germ cell tumours / Baldini, Enke; Y., Arlot Bonnemains; M., Mottolese; S., Sentinelli; B., Antoniani; Sorrenti, Salvatore; Salducci, Mauro; E., Comini; Ulisse, Salvatore; D'Armiento, Massimino. - In: ANDROLOGIA. - ISSN 0303-4569. - 42:4(2010), pp. 260-267. [10.1111/j.1439-0272.2009.00987.x]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/228845
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