Free cysteamine levels in mouse tissues have been strictly correlated to the presence of membrane-bound pantetheinase activity encoded by Vanin-1. Vanin-1 is involved in many biological processes in mouse, from thymus homing to sexual development. Vanin-1(-/-) mice are fertile and grow and develop normally, they better control inflammation and most of the knockout effects were rescued by cystamine treatment. Gene structure analysis showed the presence of an oxidative stimuli-responsive ARE-like sequence in the promoter. In this paper we investigate antioxidant-detoxifying enzymatic activities at the tissue level, comparing Vanin-1(-/-) and wild-type mice. In Vanin-1 null animals we pointed out a decrease in the Se-independent glutathione peroxidase activity. The decrease in enzymatic activity appeared to be correlated to an impairment of GST isoenzyme levels. In particular a significant drop in GSTA3 together with a minor decrement in GSTM1 and an increase in GSTP1 levels was detected in Vanin-1(-/-) livers. Cystamine administration to Vanin-1(-/-) mice restored specifically GSTA3 levels and the corresponding enzymatic activity without influencing protein expression. A possible role of cystamine on protein stability/folding can be postulated. (c) 2007 Elsevier Inc. All rights reserved.
Cystamine restores GSTA3 levels in Vanin-1 null mice / Luana Di, Leandro; Maras, Bruno; Schinina', Maria Eugenia; Silvestro, Dupre; Ilias, Koutris; Florent M., Martin; Philippe, Naquet; Franck, Galland; Giuseppina, Pitari. - In: FREE RADICAL BIOLOGY & MEDICINE. - ISSN 0891-5849. - 44:6(2008), pp. 1088-1096. [10.1016/j.freeradbiomed.2007.12.015]
Cystamine restores GSTA3 levels in Vanin-1 null mice
MARAS, Bruno;SCHININA', Maria Eugenia;
2008
Abstract
Free cysteamine levels in mouse tissues have been strictly correlated to the presence of membrane-bound pantetheinase activity encoded by Vanin-1. Vanin-1 is involved in many biological processes in mouse, from thymus homing to sexual development. Vanin-1(-/-) mice are fertile and grow and develop normally, they better control inflammation and most of the knockout effects were rescued by cystamine treatment. Gene structure analysis showed the presence of an oxidative stimuli-responsive ARE-like sequence in the promoter. In this paper we investigate antioxidant-detoxifying enzymatic activities at the tissue level, comparing Vanin-1(-/-) and wild-type mice. In Vanin-1 null animals we pointed out a decrease in the Se-independent glutathione peroxidase activity. The decrease in enzymatic activity appeared to be correlated to an impairment of GST isoenzyme levels. In particular a significant drop in GSTA3 together with a minor decrement in GSTM1 and an increase in GSTP1 levels was detected in Vanin-1(-/-) livers. Cystamine administration to Vanin-1(-/-) mice restored specifically GSTA3 levels and the corresponding enzymatic activity without influencing protein expression. A possible role of cystamine on protein stability/folding can be postulated. (c) 2007 Elsevier Inc. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
