Designer multicomponent lipoplexes have recently emerged as especially promising transfection candidates, since they are from 10 to 100 times more efficient than binary complexes usually employed for gene delivery purposes. Here, we show, for the first time, that after internalization binary complexes of lower transfection potency remain in compact perinuclear endosomes, while multicomponent systems have intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes with extremely high efficiency. Endosomal rupture results in an extraordinarily homogeneous distribution of unbound plasmid DNA throughout the cytoplasm and in the nucleus.

Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes / Caracciolo, Giulio; Caminiti, Ruggero; Pozzi, Daniela; M. A., Digman; E., Gratton; S., Sanchez; C., Marchini; M., Montani; A., Amici; H., Amenitsch. - In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. - ISSN 0175-7571. - 38 (Suppl. 1):(2009), p. S85. ((Intervento presentato al convegno European Biophysics Congress tenutosi a Genova; Italy.

Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes.

CARACCIOLO, Giulio;CAMINITI, Ruggero;POZZI, DANIELA;
2009

Abstract

Designer multicomponent lipoplexes have recently emerged as especially promising transfection candidates, since they are from 10 to 100 times more efficient than binary complexes usually employed for gene delivery purposes. Here, we show, for the first time, that after internalization binary complexes of lower transfection potency remain in compact perinuclear endosomes, while multicomponent systems have intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes with extremely high efficiency. Endosomal rupture results in an extraordinarily homogeneous distribution of unbound plasmid DNA throughout the cytoplasm and in the nucleus.
2009
European Biophysics Congress
efficient; endosomes; lipoplexes
04 Pubblicazione in atti di convegno::04c Atto di convegno in rivista
Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes / Caracciolo, Giulio; Caminiti, Ruggero; Pozzi, Daniela; M. A., Digman; E., Gratton; S., Sanchez; C., Marchini; M., Montani; A., Amici; H., Amenitsch. - In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. - ISSN 0175-7571. - 38 (Suppl. 1):(2009), p. S85. ((Intervento presentato al convegno European Biophysics Congress tenutosi a Genova; Italy.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11573/227883
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