Designer multicomponent lipoplexes have recently emerged as especially promising transfection candidates, since they are from 10 to 100 times more efficient than binary complexes usually employed for gene delivery purposes. Here, we show, for the first time, that after internalization binary complexes of lower transfection potency remain in compact perinuclear endosomes, while multicomponent systems have intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes with extremely high efficiency. Endosomal rupture results in an extraordinarily homogeneous distribution of unbound plasmid DNA throughout the cytoplasm and in the nucleus.
Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes / Caracciolo, Giulio; Caminiti, Ruggero; Pozzi, Daniela; M. A., Digman; E., Gratton; S., Sanchez; C., Marchini; M., Montani; A., Amici; H., Amenitsch. - In: EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS. - ISSN 0175-7571. - 38 (Suppl. 1):(2009), p. S85. (Intervento presentato al convegno European Biophysics Congress tenutosi a Genova; Italy).
Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes.
CARACCIOLO, Giulio;CAMINITI, Ruggero;POZZI, DANIELA;
2009
Abstract
Designer multicomponent lipoplexes have recently emerged as especially promising transfection candidates, since they are from 10 to 100 times more efficient than binary complexes usually employed for gene delivery purposes. Here, we show, for the first time, that after internalization binary complexes of lower transfection potency remain in compact perinuclear endosomes, while multicomponent systems have intrinsic endosomal rupture properties that allow plasmid DNA to escape from endosomes with extremely high efficiency. Endosomal rupture results in an extraordinarily homogeneous distribution of unbound plasmid DNA throughout the cytoplasm and in the nucleus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.