We have applied electrophoresis on agarose gels to investigate the DNA-binding capacity of cationic liposomes made of cationic DC-cholesterol and neutral dioleoylphosphatidylethanolamine as a function of membrane charge density and cationic lipid/DNA charge ratio. While each cationic liposome formulation exhibits a distinctive DNA-protection ability, here we show that such a capacity is universally regulated by surface area of lipid membranes available for binding in an aspecific manner. The relevance of DNA protection for gene transfection is also discussed.
Surface area of lipid membranes regulates the DNA-binding capacity of cationic liposomes / Marchini, C; Montani, M; Amici, A; Pozzi, Daniela; Caminiti, Ruggero; Caracciolo, Giulio. - In: APPLIED PHYSICS LETTERS. - ISSN 0003-6951. - 94:(2009), pp. 033903-1-033903-3. [10.1063/1.3074444]
Surface area of lipid membranes regulates the DNA-binding capacity of cationic liposomes
POZZI, DANIELA;CAMINITI, Ruggero;CARACCIOLO, Giulio
2009
Abstract
We have applied electrophoresis on agarose gels to investigate the DNA-binding capacity of cationic liposomes made of cationic DC-cholesterol and neutral dioleoylphosphatidylethanolamine as a function of membrane charge density and cationic lipid/DNA charge ratio. While each cationic liposome formulation exhibits a distinctive DNA-protection ability, here we show that such a capacity is universally regulated by surface area of lipid membranes available for binding in an aspecific manner. The relevance of DNA protection for gene transfection is also discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
