Bioelectrochemical properties of Trametes versicolor Laccase (TvL) and Trametes hirsuta Laccase (ThL) immobilized by using polyazetidine prepolymer (PAP) onto multi-walled carbon nanotubes (MWCNTs) screen printed electrode (SPE) surface, have been studied with several redox mediators by cyclic voltammetry (CV). The efficient entrapment of lactase in the PAP layer was confirmed by determination of both kinetic parameters (maximum current and Michaelis-Menten apparent constant) and analytical performances by chronoamperometry. The Laccase-modified MWCNTs electrode provides an effective biosensor for determination of polyphenols and catecholamines in real matrices; performances of the considered biosensors for real samples analysis are also compared and discussed.
Laccase-Polyazetidine Prepolymer-MWCNT integrated system: Biochemical properties and application to analytical determinations in real samples / Tortolini, Cristina; DI FUSCO, Massimo; Frasconi, Marco; Favero, Gabriele; Mazzei, Franco. - In: MICROCHEMICAL JOURNAL. - ISSN 0026-265X. - 96:2(2010), pp. 301-307. [10.1016/j.microc.2010.05.004]
Laccase-Polyazetidine Prepolymer-MWCNT integrated system: Biochemical properties and application to analytical determinations in real samples
TORTOLINI, CRISTINA;DI FUSCO, Massimo;FRASCONI, Marco;FAVERO, Gabriele;MAZZEI, Franco
2010
Abstract
Bioelectrochemical properties of Trametes versicolor Laccase (TvL) and Trametes hirsuta Laccase (ThL) immobilized by using polyazetidine prepolymer (PAP) onto multi-walled carbon nanotubes (MWCNTs) screen printed electrode (SPE) surface, have been studied with several redox mediators by cyclic voltammetry (CV). The efficient entrapment of lactase in the PAP layer was confirmed by determination of both kinetic parameters (maximum current and Michaelis-Menten apparent constant) and analytical performances by chronoamperometry. The Laccase-modified MWCNTs electrode provides an effective biosensor for determination of polyphenols and catecholamines in real matrices; performances of the considered biosensors for real samples analysis are also compared and discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
