A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Glil expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.
Hedgehog Signaling during Expansion of Human Pancreatic Islet-Derived Precursors / Rita, G., Fabio Arturo, G., Lorella, M., Ferretti, E., Gulino, A., Marchetti, P., Francesco, D.. - In: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES. - ISSN 0077-8923. - STAMPA. - 1150:1(2008), pp. 43-45. (9th International Congress of the Immunology-of-Diabeties-Society/American-Diabeties-Association-Research Symposium Miami Beach, FL NOV 14-18, 2007) [10.1196/annals.1447.024].
Hedgehog Signaling during Expansion of Human Pancreatic Islet-Derived Precursors
FERRETTI, ELISABETTA;GULINO, Alberto;MARCHETTI, PAOLO;
2008
Abstract
A detailed understanding of the molecular process involved in the proliferation of pancreatic precursor cells would provide key elements for developing new therapeutic strategies to cure type 1 diabetes. In the present study we investigated the potential involvement of hedgehog signaling in proliferating human pancreatic islet-derived mesenchymal (hPIDM) cells, a population of cells that can be successfully expanded and induced to differentiate into an insulin-secreting phenotype. Here we report that in these precursor cells a hedgehog signaling pathway is activated, as shown by Glil expression, and that a dose-dependent inhibition of such a pathway by cyclopamine results in a significant reduction of cell proliferation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
