Heparin-Toluidine Blue (C.I. Basic Blue 17) interaction was studied using UV-vis spectroscopic analysis of the titration of the functional groups present on the Polyanion chain. The study took into consideration both the pH and relative amount of the dye as a function of the Heparin repeat unit number; formation of a dye/polysaccharide aggregate was studied under the same conditions and structural models of the two types of aggregates were proposed. As a consequence of such an investigation a new Heparin determination methodology was developed.
Insight into the Heparin-Toluidine Blue (CI Basic Blue 17) interaction / D'Ilario, Lucio; Francolini, Iolanda; Martinelli, Andrea; Piozzi, Antonella. - In: DYES AND PIGMENTS. - ISSN 0143-7208. - STAMPA. - 80:(2009), pp. 343-348. [10.1016/j.dyepig.2008.07.015]
Insight into the Heparin-Toluidine Blue (CI Basic Blue 17) interaction
D'ILARIO, LUCIO;FRANCOLINI, IOLANDA;MARTINELLI, Andrea;PIOZZI, Antonella
2009
Abstract
Heparin-Toluidine Blue (C.I. Basic Blue 17) interaction was studied using UV-vis spectroscopic analysis of the titration of the functional groups present on the Polyanion chain. The study took into consideration both the pH and relative amount of the dye as a function of the Heparin repeat unit number; formation of a dye/polysaccharide aggregate was studied under the same conditions and structural models of the two types of aggregates were proposed. As a consequence of such an investigation a new Heparin determination methodology was developed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
