This is the first report on the in vitro asymbiotic germination of Orchis mascula L. seeds, collected in the Simbruini Natural Park (Italy). Different pre-inoculum treatments and various culture media were tested to induce germination and to improve embryo development. The Orchimax medium supplemented with benzyladenine and active charcoal proved to be the best in promoting seed germination. The time course of protocorm development is described.
In vitro asymbiotic germination of Orchis mascula L / Valletta, Alessio; Attorre, Fabio; Bruno, Franco; Pasqua, Gabriella. - In: PLANT BIOSYSTEMS. - ISSN 1126-3504. - STAMPA. - 142:3(2008), pp. 653-655. [10.1080/11263500802411205]
In vitro asymbiotic germination of Orchis mascula L.
VALLETTA, ALESSIOPrimo
;ATTORRE, FabioSecondo
;BRUNO, FrancoPenultimo
;PASQUA, Gabriella
2008
Abstract
This is the first report on the in vitro asymbiotic germination of Orchis mascula L. seeds, collected in the Simbruini Natural Park (Italy). Different pre-inoculum treatments and various culture media were tested to induce germination and to improve embryo development. The Orchimax medium supplemented with benzyladenine and active charcoal proved to be the best in promoting seed germination. The time course of protocorm development is described.| File | Dimensione | Formato | |
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