We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.
Surface effects on PCR reactions in multichip microfluidic platforms / Nicholas J., Panaro; Xing Jian, Lou; Fortina, Paolo; Larry J., Kricka; Peter, Wilding. - In: BIOMEDICAL MICRODEVICES. - ISSN 1387-2176. - STAMPA. - 6:1(2004), pp. 75-80. [10.1023/b:bmmd.0000013369.34716.8d]
Surface effects on PCR reactions in multichip microfluidic platforms
FORTINA, PAOLO;
2004
Abstract
We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
